Anti-RABL3 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - Q5HYI8

Core Product Specifications and Parameters

Biological Background: RABL3 Function and Localization

• RABL3 is a member of the Rab-like family of small GTPases, which play key roles in intracellular membrane trafficking and signaling.
• It functions as a critical regulator of KRAS prenylation and signaling, modulating cell proliferation. Related references: PMID:31406347
• RABL3 is required for lymphocyte development and function, but not for myeloid cell development (By similarity).
• The protein promotes ciliary vesicle formation at the mother centriole by interacting with Rab11, thereby regulating early ciliogenesis; its GTP-binding capacity is essential for this process. Related references: PMID:36052645
• Subcellularly, RABL3 localizes to the cytoplasm, centrosome (microtubule organizing center), and Golgi apparatus.
• The protein is characterized by GTP-binding, hydrolase, and metal-binding domains, and has been identified in proteomics studies as part of the reference proteome.

Experimental Guidance and Technical Tips

• The immunogen spans nearly the full-length human RABL3 protein (amino acids 1-236); this polyclonal antibody is expected to recognize multiple epitopes, enhancing signal in WB and IF/ICC.
• For Western blotting, consider using whole-cell lysates from human or mouse cell lines; the predicted molecular weight is ~26 kDa. Include a positive control known to express RABL3.
• In IF/ICC experiments, optimize fixation and permeabilization; RABL3 exhibits cytoplasmic, centrosomal, and Golgi staining, which may appear punctate or perinuclear.
• For ELISA, the recombinant immunogen can serve as a positive control to validate antibody binding and sensitivity.
• As with polyclonal antibodies, lot-to-lot variability is possible; validate each new lot in your specific assay system.
• Reported cross-reactivity with mouse RABL3 should be confirmed in your strain or model of interest.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio connects diagnostic manufacturers and researchers with a curated portfolio of IVD-grade antibodies and ancillary raw materials. This anti-RABL3 polyclonal antibody supports research into KRAS signaling, ciliogenesis, and immune cell development — key areas for oncology and rare disease diagnostics. Beyond individual antibodies, CamelBio offers validated antibody pairs, bulk monoclonal/polyclonal antibody sourcing, and technical consultations to help streamline your product development from concept to clinic.