Anti-TOR4A Monoclonal Antibody for WB, ELISA - Q9NXH8

Core Product Specifications and Parameters

Biological Background: TOR4A Function and Localization

• TOR4A (Torsin-4A), also known as Torsin family 4 member A, is encoded by the TOR4A (C9orf167) gene in humans.
• It belongs to the torsin family of AAA+ ATPases, characterized by ATP-binding and nucleotide-binding domains.
• TOR4A is a membrane protein that contains at least one transmembrane helix, as indicated by UniProt annotation.
• The protein is subject to phosphorylation, as identified through proteomics, suggesting regulation by post-translational modification.
• Subcellularly, TOR4A localizes to the membrane, consistent with its transmembrane regions.
• The molecular weight of TOR4A is approximately 47 kDa (calculated 46.9 kDa), and it is classified as a reference proteome component.

Experimental Guidance and Technical Tips

• The immunogen covers the N-terminal region (aa 1-130), which may target an exposed domain suitable for Western blotting and ELISA applications.
• For WB, consider using reducing conditions and validate antibody specificity with relevant positive controls (e.g., human or mouse cell lines expressing TOR4A).
• In ELISA, the recombinant immunogen can serve as a positive control; however, background binding should be assessed with a mismatched protein.
• Optimal antibody dilutions and incubation conditions should be determined empirically for each assay system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a streamlined procurement solution for IVD manufacturers and researchers, providing validated raw materials including this anti-TOR4A monoclonal antibody. As a dedicated partner in diagnostics development, we supply optimized monoclonal/polyclonal antibodies, recombinant proteins, and ancillary reagents to accelerate your project from concept to clinic. Contact us for bulk ordering, custom sourcing, or technical consultation.