Anti-Brachyury Rabbit Monoclonal Antibody for WB, IF/ICC, ELISA - O15178

Core Product Specifications and Parameters

Biological Background: Brachyury Function and Localization

• Brachyury (T-box transcription factor T) is a member of the T-box family of transcription factors, essential for mesoderm formation and differentiation.
• It binds to a palindromic DNA sequence (5'-TTCACACCTAGGTGTGAA-3') known as the T site, activating transcription of target genes.
• The protein is localized to the nucleus, consistent with its role as a transcription factor.
• Alternative splicing generates multiple isoforms, as indicated by UniProt keywords.
• It is classified as a developmental protein and has been linked to disease variants.
• Tissue specificity: detected in testis at protein level, but not in other normal tissues; found in lung tumors.
• The human Brachyury protein has a molecular weight of approximately 47.4 kDa (UniProt; product lists 47kDa).

Experimental Guidance and Technical Tips

• The immunogen corresponds to the C-terminal region (aa 266–435), which is likely conserved across species, explaining cross-reactivity with mouse and rat.
• For Western blotting, expect a band near 47 kDa; validate loading controls in your sample system.
• In immunofluorescence/ICC, anticipate nuclear staining; optimize fixation and permeabilization for your cell type.
• For ELISA, consider pairing with another clone or polyclonal antibody for sandwich assays if quantitative detection is needed.
• Since the antibody is validated for multiple applications, check lot-specific recommendations for working dilutions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your dedicated partner for IVD raw material sourcing, offering a comprehensive portfolio from validated monoclonal antibodies to bulk ancillary reagents. The Brachyury antibody supports developmental biology and oncology research, aligning with our commitment to delivering high-quality tools for transcription factor studies. Whether you need optimized antibody pairs, rare target reagents, or scalable supply solutions, CamelBio bridges the gap from concept to clinic with reliable procurement and technical support.