Anti-C8orf4 Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - Q9NR00

Core Product Specifications and Parameters

Biological Background: C8orf4 Function and Localization

• C8orf4, also known as Thyroid cancer protein 1 (TC-1) or TCIM, is a transcriptional and immune response regulator.
• It modulates the WNT-CTNNB1 pathway by relieving CBY1 antagonism, enhancing follicular dendritic cell proliferation. Related references: PMID:16424001, PMID:16730711
• Positively regulates G1-to-S-phase transition via the MAPK2/3 signaling pathway. Related references: PMID:18959821
• In endothelial cells, it enhances inflammatory mediators through NF-kappaB transcriptional regulation. Related references: PMID:19684084
• Plays a role in heat shock response via a positive feedback loop with HSF1. Related references: PMID:17603013
• In thyroid and lung cancers, it promotes cell proliferation and inhibits apoptosis. Related references: PMID:15087392, PMID:24941347
• Conversely, it suppresses liver cancer stem cell self-renewal by inhibiting NOTCH2 signaling. Related references: PMID:25985737
• Subcellularly, it localizes to the cytoplasm, nucleus, nucleolus, and nuclear speckles.
• Ubiquitously expressed, with elevated levels in thyroid papillary carcinoma and lung cancer, but decreased in hepatocellular carcinoma.
• The predicted molecular weight is 12.3 kDa, and the antibody recognizes a ~12 kDa band.

Experimental Guidance and Technical Tips

• Consider optimizing antibody dilution for each application; for Western blot, typical starting dilutions range from 1:500 to 1:2000.
• The immunogen covers the N-terminal region (aa 1-106); the antibody may recognize full-length C8orf4 and potential isoforms.
• For IHC-P, heat-induced antigen retrieval in citrate buffer (pH 6.0) may be tested to enhance signal.
• Validate antibody performance in your specific sample type and experimental conditions, as expression levels and modifications can vary.

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