Anti-CD152/CTLA-4 Rabbit Monoclonal Antibody for WB and ELISA - P16410

Core Product Specifications and Parameters

Biological Background: CTLA-4 Function and Localization

• CTLA-4 (Cytotoxic T-lymphocyte protein 4, CD152) is an inhibitory receptor that functions as a major negative regulator of T-cell immune responses. It binds B7 ligands CD80 and CD86 with significantly higher affinity than the stimulatory receptor CD28, thereby outcompeting CD28 and dampening T-cell activation. Related references: PMID:11279501 PMID:11279502 PMID:16551244
• Acts as a decoy receptor: by sequestering CD80/CD86, it prevents CD28-mediated co-stimulation, crucial for maintaining immune homeostasis and peripheral tolerance.
• Belongs to the immunoglobulin superfamily, featuring a single immunoglobulin V-like domain in its extracellular region.
• Predominantly localized on the cell membrane (single-pass type I transmembrane protein) and may also exist in a soluble splice variant lacking the transmembrane domain.
• Widely expressed, with highest levels in lymphoid tissues; detected on activated T-cells but at 30- to 50-fold lower surface levels compared to CD28.
• Post-translational modifications include disulfide bond formation, N-linked glycosylation, and phosphorylation, which modulate its trafficking and function.
• Genetic variants in CTLA4 are associated with susceptibility to autoimmune diseases such as type 1 diabetes and systemic lupus erythematosus, underscoring its critical role in immune regulation.

Experimental Guidance and Technical Tips

• The recombinant protein immunogen spans residues 37–162, covering the majority of the extracellular domain (excluding the signal peptide). This design may recognize both full-length membrane-bound CTLA-4 and soluble isoforms.
• For Western blot, run samples under reducing conditions; expect a band near 25 kDa. Verify specificity using positive (activated T-cell lysates) and negative controls.
• In ELISA applications, consider using the antibody as a capture or detection reagent. Due to low native expression, sample enrichment (e.g., immunoprecipitation) or high-sensitivity substrates may be necessary.
• Cross-reactivity data is limited to human; for non-human samples, validate binding beforehand.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio partners with IVD manufacturers and research laboratories by providing reliable, one-stop access to critical raw materials. For CTLA-4 immunoassay development, we supply validated monoclonal and polyclonal antibodies, matched antibody pairs, and ancillary reagents. Our global supply network includes rare-target raw material sourcing and bulk-scale support, helping you move efficiently from concept to clinical application.