Anti-TOMM40 Monoclonal Antibody for WB - O96008

Core Product Specifications and Parameters

Biological Background: TOMM40 Function and Localization

• TOMM40 is a channel-forming protein and a core subunit of the translocase of the outer mitochondrial membrane (TOM) complex, facilitating recognition and import of nuclear-encoded mitochondrial preproteins. Related references: PMID:15644312, PMID:31206022, PMID:40080546
• Upon mitochondrial depolarization, TOMM40 interacts with VDAC2 and PINK1 kinase to stabilize PINK1 on the outer membrane, initiating PRKN-dependent mitophagy. Related references: PMID:40080546
• It contributes to the assembly of mitochondrial respiratory Complex I by partnering with BCAP31 to mediate cytosolic-to-mitochondrial translocation of Complex I components. Related references: PMID:31206022
• Localized to the mitochondrial outer membrane, TOMM40 functions as a beta-barrel porin involved in ion and protein transport.
• Alternative splicing generates multiple TOMM40 isoforms, extending functional diversity.
• Keywords for TOMM40 include ion transport, protein transport, transmembrane beta strand, and alternative splicing.

Experimental Guidance and Technical Tips

• The immunogen is the N-terminal 1–90 amino acids of human TOMM40. For applications requiring detection of C-terminal variants or isoforms, alternative antibodies may be needed.
• For Western blotting, the expected band is approximately 38 kDa; use appropriate molecular weight markers and verify in the relevant sample system.
• When using in immunohistochemistry (IHC-P) or immunofluorescence (IF/ICC), optimize fixation and antigen retrieval conditions to preserve epitope recognition.
• This antibody is reported to detect human, mouse, and rat TOMM40; validate cross-reactivity in your specific experimental context.
• For ELISA or immunoprecipitation, titrate antibody concentration and include appropriate controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers comprehensive raw-material sourcing solutions for diagnostic and research applications. This TOMM40 antibody supports studies in mitochondrial import, mitophagy, and respiratory complex assembly. We provide validated antibodies, antigen pairs, and bulk reagents to streamline your assay development and procurement needs.