Anti-TRAF4 Rabbit Monoclonal Antibody for WB, ELISA - Q9BUZ4

Core Product Specifications and Parameters

Biological Background: TRAF4 Function and Localization

• TRAF4 is an adapter protein and E3 ubiquitin ligase involved in diverse biological processes including cell proliferation, migration, differentiation, DNA repair, platelet activation, and apoptosis. Related references: PMID:30352854, PMID:31076633, PMID:32268273
• It promotes EGFR-mediated signaling by facilitating EGFR dimerization and downstream AKT activation, enhancing cell proliferation. Related references: PMID:30352854
• TRAF4 ubiquitinates SMURF2 via Lys-48-linked chains, targeting it for proteasomal degradation and thereby promoting osteogenic differentiation. Related references: PMID:31076633
• Through Lys-63-linked ubiquitination of CHK1, TRAF4 activates cell cycle arrest and DNA repair pathways. Related references: PMID:32357935
• It catalyzes atypical Lys-29-linked ubiquitination of IRS1, which is crucial for insulin-like growth factor (IGF) signal transduction. Related references: PMID:33991522
• TRAF4 inhibits adipogenic differentiation by activating pyruvate kinase PKM and the beta-catenin signaling pathway. Related references: PMID:32268273
• It also regulates NF-kappa-B activation downstream of Toll-like receptors and is required for normal skeleton and respiratory tract development (By similarity).
• TRAF4 localizes to multiple subcellular compartments: cytoplasm (including perinuclear region), nucleus, tight junctions, cell membrane, and cytoskeleton.
• Tissue expression is prominent in epithelial cells of the thymus, dendritic cells of lymph nodes, and basal epithelial layers of the epidermis, nasopharynx, respiratory tract, salivary gland, and esophagus.
• The protein contains characteristic RING-type zinc finger and TRAF domains, along with a coiled-coil region, and is subject to phosphorylation and ubiquitination.

Experimental Guidance and Technical Tips

• The immunogen encompasses the C-terminal region (aa 209-470) containing the TRAF domain, which is highly conserved across human, mouse, and rat, consistent with the antibody’s cross-reactivity.
• For Western blotting, consider using whole-cell lysates from TRAF4-expressing cell lines (e.g., HeLa, HEK293) and validate with positive and negative controls. The predicted molecular weight is 54 kDa, but post-translational modifications may result in slight band shifts.
• In ELISA assays, this monoclonal antibody can serve as either a capture or detection reagent; it is advisable to perform a titration to determine the optimal working concentration for your specific assay format.
• Always include appropriate controls and optimize experimental conditions for your sample type and application.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your integrated sourcing partner for IVD raw materials, supplying high-quality antibodies, antigens, and ancillary reagents to diagnostic manufacturers and research laboratories worldwide. This anti-TRAF4 monoclonal antibody supports investigations into cell signaling, cancer biology, and developmental pathways. Whether you need validated antibody pairs, bulk monoclonal antibodies, or custom raw-material sourcing for rare targets, CamelBio provides reliable, one-stop access from concept to clinic.