Anti-eIF4E Rabbit Monoclonal Antibody [KD Validated] for WB, IF/ICC, IP, ELISA - P06730

Core Product Specifications and Parameters

Biological Background: eIF4E Function and Localization

• Eukaryotic translation initiation factor 4E (eIF4E), also known as eIF4E1, eIF-4F 25 kDa subunit, or mRNA cap-binding protein, is encoded by the EIF4E gene (Gene ID: 1977). It serves dual functions in the cytoplasm for translation initiation and in the nucleus for mRNA export (PubMed:11606200, PubMed:22578813, PubMed:22684010).
  Related references:
  PMID:11606200, PMID:22578813, PMID:22684010

• eIF4E specifically recognizes and binds the 7-methylguanosine (m7G) cap at the 5' end of mRNAs, a critical step that facilitates ribosome recruitment and unwinding of mRNA secondary structures (PubMed:16271312, PubMed:22578813). Together with EIF4G1, it enables TISU element-dependent translation, bypassing the need for ribosomal scanning (PubMed:29987188).
  Related references:
  PMID:16271312, PMID:22578813, PMID:29987188

• Beyond initiation, eIF4E regulates cytoplasmic mRNA stability and storage. In P-bodies, it participates in a complex that sequesters translationally repressed mRNAs, preventing their degradation (PubMed:24335285).
  Related references:
  PMID:24335285

• eIF4E facilitates the nuclear export of a subset of mRNAs, including NOS2/iNOS and MDM2, through interactions with the 4ESE element and the LRPPRC–XPO1 export pathway, thereby influencing processes such as apoptosis and cell cycle progression (PubMed:23471078, PubMed:22684010).
  Related references:
  PMID:23471078, PMID:22684010

• Subcellularly, eIF4E localizes to the cytoplasm, P-bodies, stress granules, as well as the nucleus (including nuclear speckles and nuclear bodies), reflecting its multifaceted roles.

• eIF4E is subject to phosphorylation (e.g., at Ser209) which modulates its cap-binding affinity and translation activity. Acetylation has also been reported, and the protein undergoes alternative splicing, yielding isoforms with molecular masses around 25–29 kDa.

Experimental Guidance and Technical Tips

• The immunogen, a synthetic peptide spanning residues 1–100 of human eIF4E (NP_001959.1), targets the conserved N‑terminal region. Based on the sequence homology, cross‑reactivity with mouse, rat, and monkey orthologs is anticipated, as confirmed by the supplier.
• In western blot (WB), expect bands corresponding to the 25 kDa, 27 kDa, and 29 kDa isoforms; loading controls and blocking conditions should be optimized for the sample type.
• For immunofluorescence (IF/ICC), eIF4E typically displays a diffuse cytoplasmic signal with punctate nuclear staining; fixation and permeabilization protocols may need adjustment depending on the cell line.
• This antibody is validated for immunoprecipitation (IP) and ELISA; for quantitative assays, include appropriate positive and negative controls.
• Because eIF4E participates in multiple complexes, native IP may co‑precipitate interaction partners; denaturing lysis conditions can be used to isolate total eIF4E.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop partner for IVD raw materials, connecting diagnostic manufacturers and research laboratories with critical reagents. This anti-eIF4E rabbit monoclonal antibody exemplifies our commitment to supplying high-quality, validated antibodies for translation initiation and mRNA metabolism studies. From bulk ancillary reagents to customized monoclonal antibody production and sourcing of rare targets, our integrated services support you from concept to clinic.