Anti-SUMO4 Rabbit Monoclonal Antibody for WB, IHC-P, IF/ICC, ELISA - Q6EEV6

Core Product Specifications and Parameters

Biological Background: SUMO4 Function and Localization

• SUMO4 is a ubiquitin-like protein that is covalently attached to target lysines as a monomer, modulating protein localization, stability, or activity.
• Under oxidative stress, SUMO4 conjugates to various antioxidant enzymes, chaperones, and stress defense proteins.
• SUMO4 negatively regulates transcriptional activity of NFKBIA, TFAP2A, and FOS, and positively regulates NR3C1.
• Covalent attachment requires prior activation by the E1 complex SAE1-SAE2 and linkage to the E2 enzyme UBE2I.
• Expressed mainly in adult and embryonic kidney, with highest immune tissue expression in lymph node and spleen.
• Associated with diabetes mellitus (IDDM5); contains isopeptide bonds and participates in the Ubl conjugation pathway.

Experimental Guidance and Technical Tips

• For Western blot (WB), the predicted molecular weight is 11 kDa. Use reducing conditions and consider positive controls such as HEK293 cell lysates or immune tissue extracts.
• For immunohistochemistry (IHC-P), validate in kidney or lymph node sections, as these tissues show high SUMO4 expression.
• For immunofluorescence (IF/ICC), confirm subcellular localization, considering that SUMO4 conjugation may alter target distribution.
• Due to the small size, use appropriate gel percentage and transfer conditions; low molecular weight markers are recommended.
• Cross-reactivity with human, mouse, and rat has been reported; validate in the relevant species model.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a one-stop provider of IVD raw materials, technical services, and consulting tailored for diagnostic manufacturers and research laboratories. This anti-SUMO4 monoclonal antibody supports studies into SUMOylation, oxidative stress, and diabetes. Beyond antibodies, CamelBio supplies validated antibody pairs, bulk ancillary reagents, and customized sourcing for rare targets—ensuring your projects move seamlessly from concept to clinic.