Anti-TorsinA/TOR1A Rabbit Monoclonal Antibody for WB, IF/ICC, ELISA - O14656

Core Product Specifications and Parameters

Biological Background: TorsinA/TOR1A Function and Localization

• Torsin-1A, also known as Dystonia 1 protein or TOR1A, is encoded by the TOR1A gene (GeneID: 1861) and belongs to the torsin ATPase family.
• Functions as a molecular chaperone, controlling protein folding, processing, stability, and localization; helps reduce misfolded protein aggregates.
• Regulates synaptic vesicle recycling by stabilizing STON2 in collaboration with the COP9 signalosome complex.
• Links the cytoskeleton to the nuclear envelope, crucial for nuclear polarity, cell movement, and neuronal nuclear envelope integrity.
• Modulates subcellular localization of multipass membrane proteins like dopamine transporter SLC6A3, thereby influencing dopamine neurotransmission.
• Localizes to the endoplasmic reticulum lumen, nucleus membrane, cell projections (growth cones), cytoplasmic vesicles (secretory, synaptic), and the cytoskeleton.
• Widely expressed with highest levels in kidney, liver, and brain; prominently expressed in dopaminergic neurons of substantia nigra, neocortex, hippocampus, cerebellum, and spinal cord.
• Post-translationally modified as a glycoprotein; mutations in TOR1A cause early-onset torsion dystonia (DYT1).

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 233–332 within the C-terminal region of human TorsinA; verify epitope accessibility in your specific assay format.
• For Western blot, expect a band near 38 kDa under reducing conditions; consider using positive controls derived from tissues with high TorsinA expression (e.g., kidney, brain, liver).
• In IF/ICC, permeabilization is essential for detecting nuclear membrane and ER-localized pools; co-stain with organelle markers to confirm localization.
• ELISA applications may benefit from a peptide competition assay to confirm signal specificity.
• Cross-reactivity with mouse and rat is reported; validate the antibody in your model system to ensure consistent performance.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research laboratories with validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents. For TorsinA/TOR1A-related studies in neuroscience and protein misfolding disorders, we offer one-stop access to IVD raw materials, technical consultation, and rare-target sourcing. Partner with us to streamline your development pipeline from concept to clinic.