Anti-GATA4 Rabbit Monoclonal Antibody for WB, IF/ICC, IHC-P, ELISA - P43694

Core Product Specifications and Parameters

Biological Background: GATA4 Function and Localization

• GATA4 is a zinc-finger transcription factor that binds the 5'-AGATAG-3' motif and plays a pivotal role in cardiac development and function. It cooperates with TBX5 to bind cardiac super-enhancers, promoting cardiomyocyte gene expression and repressing endocardial/endothelial genes, and participates in BMP-mediated cardiac gene induction. Related references: PMID:24000169 PMID:27984724 PMID:35182466
• GATA4 promotes cardiac myocyte enlargement. Related references: PMID:20081228
• GATA4 is required during testicular development. Related references: PMID:21220346
• GATA4 may regulate sphingolipid signaling by controlling sphingosine-1-phosphate lyase expression. Related references: PMID:15734735
• GATA4 localizes to the nucleus, consistent with its role as a transcription factor.
• Mutations in GATA4 are associated with congenital heart diseases including atrial septal defect and cardiomyopathy.
• GATA4 undergoes post-translational modifications such as methylation, which may regulate its transcriptional activity.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a peptide within amino acids 343-442 of human GATA4, suggesting the antibody may recognize epitopes in this C-terminal region. For Western blot (WB) and other applications, consider using whole cell lysates from human, mouse, or rat cardiac or testicular tissues given the reported cross-reactivity and expression patterns.
• For immunofluorescence (IF/ICC) and immunohistochemistry (IHC-P), nuclear localization of GATA4 should be observed; perform appropriate fixation and permeabilization. Validate in the relevant sample system based on your experimental context.
• As with any antibody, titrate and optimize working dilutions for your specific assay conditions and sample types.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and researchers with streamlined access to high-quality IVD antibodies like anti-GATA4. We support cardiac biomarker and transcription factor research with validated antibody pairs, bulk monoclonal antibodies, and ancillary reagents, helping you accelerate assay development from concept to clinic. Our comprehensive sourcing covers rare targets and custom development needs tailored to your project.