Anti-TERT Monoclonal Antibody for WB, ELISA - O14746

Core Product Specifications and Parameters

Biological Background: TERT Function and Localization

• Telomerase reverse transcriptase (TERT) is the catalytic subunit of the telomerase ribonucleoprotein complex, essential for de novo telomere repeat addition to chromosome ends.
• The enzyme cycles through primer binding, nucleotide addition, translocation, and release, adding the hexameric repeat 5’-TTAGGG-3’; it is active mainly in stem cells, germ cells, and the majority of cancers, while silenced in most normal somatic tissues.
• Beyond telomere elongation, TERT modulates Wnt signaling and influences cellular aging and anti-apoptotic pathways.
• Multiple isoforms arise from alternative splicing; the full-length protein is predominantly nuclear, localizing to nucleoli, nucleoplasm, and telomeres, but is also detected in cytoplasmic PML bodies.
• TERT expression is tissue-specific: highest in thymocyte subpopulations, intermediate in tonsillar T-lymphocytes, and low to undetectable in peripheral blood T-cells.
• The protein is subject to phosphorylation and functions as a DNA-binding, metal-dependent nucleotidyltransferase, requiring magnesium for catalysis.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the C-terminal region (amino acids 1000–1132) of human TERT, which is well-conserved across human, mouse, and rat, supporting the observed cross-reactivity.
• For Western blotting, expect a band near 127 kDa; additional lower bands may indicate splice variants or degradation. Include appropriate positive controls (e.g., HeLa cells, mouse testis lysate) and validate under your experimental conditions.
• In ELISA, consider using recombinant TERT protein or the immunogen as a coating antigen; both indirect and sandwich formats may be suitable.
• Because TERT expression is often low, enrichment (e.g., nuclear extracts) or signal amplification may enhance detection sensitivity.
• While cross-reactivity with mouse and rat is indicated, always confirm performance in your specific sample system by testing appropriate positive and negative controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio partners with diagnostic manufacturers and research institutes to streamline the supply of critical IVD raw materials. This TERT monoclonal antibody is a key resource for oncology, aging, and telomere biology research. From validated antibody pairs and optimized monoclonal/polyclonal antibodies to bulk ancillary reagents, we provide one-stop access to accelerate your assay development and commercialization.