Anti-CD40 Rabbit Monoclonal Antibody for WB and ELISA - P25942

Core Product Specifications and Parameters

Biological Background: CD40 Function and Localization

• Official name: Tumor necrosis factor receptor superfamily member 5; also known as B-cell surface antigen CD40, Bp50, CD40L receptor, and CDw40.
• CD40 acts as a receptor for CD40 ligand (TNFSF5/CD40LG).
  Related references: PMID:31331973
• Ligand binding triggers TRAF6- and MAP3K8-mediated ERK activation in macrophages and B cells, leading to induction of immunoglobulin secretion.
• Alternative splicing yields multiple isoforms; the canonical isoform is a single-pass type I membrane protein.
• Localizes primarily to the cell membrane, with a secreted form also reported.
• Highly expressed in B lymphocytes and in primary carcinomas.
• The extracellular domain contains several disulfide bonds and glycosylation sites.
• Key biological roles: immune response, B cell activation, and co-stimulation; genetic variants are associated with disease.

Experimental Guidance and Technical Tips

• The immunogen (aa 21–193) covers the extracellular domain; the antibody likely recognizes the extracellular region of CD40.
• For Western blotting, expect a band near 31–40 kDa due to post-translational modifications; optimize sample preparation and gel conditions for membrane proteins.
• In ELISA, consider using this antibody as a capture or detection reagent in a matched-pair format; cross-reactivity with other TNFR family members should be assessed.
• Validate antibody performance in your specific sample system (e.g., B-cell lines, tumor biopsies) with appropriate controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and researchers with a streamlined one-stop sourcing platform for IVD raw materials, including validated antibody pairs, optimized monoclonal/polyclonal antibodies, and ancillary bulk reagents. For targets like CD40, a critical immune checkpoint and B-cell marker, CamelBio supports procurement of rare-target raw materials and technical consulting to accelerate assay development from concept to clinic.