Anti-THBS1 Polyclonal Antibody for WB, IHC-P, ELISA - P07996

Core Product Specifications and Parameters

Biological Background: THBS1 Function and Localization

• Thrombospondin-1 (THBS1, glycoprotein G) is an adhesive glycoprotein mediating cell-to-cell and cell-to-matrix interactions, critical for tissue integrity and repair (PubMed:15014436, PubMed:18285447, PubMed:2430973). Related references: PMID:15014436, PMID:18285447, PMID:2430973
• Multifunctional protein broadly involved in inflammation, angiogenesis, wound healing, ROS and NO signaling, apoptosis, senescence, stem cell self-renewal, and metabolic homeostasis (PubMed:10613822, PubMed:11134179, PubMed:1371676). Related references: PMID:10613822, PMID:11134179, PMID:1371676
• Negatively modulates dendritic cell activation and cytokine release, contributing to immune homeostasis and resolution of inflammation (PubMed:14568985). Related references: PMID:14568985
• Acts as a ligand for CD47, modulating nitric oxide signaling, supporting blood pressure, and promoting endothelial cell senescence via NOX1/ROS (PubMed:19004835, PubMed:8550562, PubMed:29042481). Related references: PMID:19004835, PMID:8550562, PMID:29042481
• Binds CD36 to induce apoptosis in podocytes and suppress angiogenesis, depending on cellular context (PubMed:10613822, PubMed:11134179, PubMed:1371676). Related references: PMID:10613822, PMID:11134179, PMID:1371676
• Interacts with SIRPA to stimulate NADPH oxidase-derived ROS production in non-phagocytic cells (PubMed:24511121). Related references: PMID:24511121
• Secreted protein that localizes to the cell surface, extracellular matrix, endoplasmic reticulum, and sarcoplasmic reticulum.
• Expressed by platelets and monocyte-derived dendritic cells (PubMed:101549, PubMed:14568985). Related references: PMID:101549, PMID:14568985

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 19-352 of human THBS1, covering the N-terminal region; consider its suitability for detecting full-length protein and potential cleavage products.
• For Western blot, note the predicted molecular weight of 129 kDa; optimize denaturing conditions and use appropriate molecular weight markers.
• For immunohistochemistry on paraffin-embedded sections, validate antibody specificity in tissues known to express THBS1.
• Cross-reactivity is reported with human, mouse, and rat; verify performance in the relevant species and experimental context.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research labs with one-stop access to high-quality IVD raw materials. For studies of THBS1 in angiogenesis, inflammation, and metabolic disorders, we offer validated polyclonal and monoclonal antibodies, optimized antibody pairs, and bulk ancillary reagents to support your assay development. Whether you need rare target raw materials or comprehensive technical consulting, CamelBio bridges your project from concept to clinic.