Anti-TSHB Rabbit Polyclonal Antibody for IHC-P, IF/ICC, ELISA - P01222

Core Product Specifications and Parameters

Biological Background: Thyrotropin subunit beta Function and Localization

• The TSHB gene encodes the beta subunit of thyroid-stimulating hormone (TSH), a key pituitary glycoprotein hormone.
• TSH is indispensable for the control of thyroid structure and metabolism, regulating thyroid gland activity.
• The mature TSH hormone is a heterodimer composed of a common alpha subunit and a hormone-specific beta subunit; TSHB confers thyroid specificity.
• TSH is secreted and travels through the bloodstream to act on the thyroid gland, stimulating hormone synthesis and release.
• Post-translational modifications include glycosylation and formation of intramolecular disulfide bonds, critical for proper folding and bioactivity.
• Alternative splicing of TSHB produces multiple transcript variants, potentially contributing to functional diversity.
• Mutations in TSHB are associated with congenital hypothyroidism, a condition of inadequate thyroid hormone production.
• The mature TSHB protein has a predicted molecular weight of approximately 15.6 kDa, often observed at ~16 kDa on SDS-PAGE due to glycosylation.

Experimental Guidance and Technical Tips

• For IHC-P, antigen retrieval may be necessary; validate on thyroid or pituitary tissue sections as positive controls.
• In IF/ICC, expect cytoplasmic or secretory vesicle staining; use appropriate permeabilization and include negative controls.
• For ELISA, the antibody can serve as a detection reagent; determine optimal coating concentration and consider pairing with a matched capture antibody for sandwich assays.
• Store at -20°C upon arrival; minimize freeze-thaw cycles to maintain activity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research labs with a reliable one-stop sourcing solution for IVD raw materials. This anti-TSHB polyclonal antibody supports thyroid biomarker assay development, and we offer complementary matched antibody pairs, recombinant antigens, and bulk reagent options. Let CamelBio streamline your supply chain from target selection to clinical validation.