Anti-Thyroid Stimulating Hormone (TSH) Polyclonal Antibody for IHC-P, ELISA - P01215 / P01222

Core Product Specifications and Parameters

Biological Background: Thyroid Stimulating Hormone Function and Localization

• Thyroid Stimulating Hormone (TSH) is a heterodimeric glycoprotein hormone composed of an alpha chain (CGA, GeneID: 1081) and a beta chain (TSHB, GeneID: 7252).
• The alpha subunit is shared with other anterior pituitary glycoprotein hormones including LH, FSH, and CG, while the beta subunit confers hormone-specific biological activity and receptor binding.
• TSH binds to the TSH receptor (TSHR) on thyroid follicular cells, activating adenylate cyclase and stimulating the production and secretion of thyroid hormones (T3 and T4).
• Both alpha and beta chains are secreted from thyrotrophs in the anterior pituitary, with secretion regulated by thyrotropin-releasing hormone (TRH) and negative feedback from thyroid hormones.
• The alpha chain contains multiple N-linked glycosylation sites and disulfide bonds essential for correct folding, subunit assembly, and hormone stability.
• Structural studies have defined the cysteine-knot motif common to glycoprotein hormones and the heterodimer interface critical for receptor interaction.
• Keywords associated with the alpha chain include glycoprotein, disulfide bond, signal peptide, and secretion, reflecting its processed and secreted nature.

Experimental Guidance and Technical Tips

• The immunogen covers sequences from both the alpha (aa 25-116) and beta (aa 21-138) subunits, which may yield antibodies recognizing the mature heterodimer and potentially individual chains.
• For IHC-P, antigen retrieval conditions and antibody dilution should be optimized; positive control tissues include human or mouse pituitary gland and thyroid tissue.
• In ELISA, consider pairing this polyclonal antibody as the capture or detection reagent; cross-reactivity to mouse TSH (as noted) supports utility in preclinical models.
• Due to the shared alpha chain, some cross-reactivity with other glycoprotein hormones (LH, FSH, CG) is possible in samples containing high levels of these hormones; validate specificity in the intended sample matrix.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a dedicated partner for diagnostic manufacturers and research laboratories, providing one-stop access to IVD raw materials and technical support from concept to clinic. In the area of endocrine diagnostics, we offer validated antibody pairs, optimized polyclonal and monoclonal antibodies, bulk ancillary reagents, and rare target sourcing—enabling streamlined development of thyroid function assays and related biomarker tests.