Anti-TPMT Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - P51580

Core Product Specifications and Parameters

Biological Background: TPMT Function and Localization

• TPMT (Thiopurine S-methyltransferase), also known as thiopurine methyltransferase, catalyzes the S-methylation of thiopurine drugs such as 6-mercaptopurine and 6-thioguanine using S-adenosyl-L-methionine as the methyl donor (PubMed:18484748, PubMed:657528). Related references: PMID:18484748 PMID:657528
• The enzyme plays a critical role in modulating the cytotoxic effects of thiopurine prodrugs, which is clinically significant for dosing and toxicity in leukemia and autoimmune disease treatments.
• No natural endogenous substrate has been identified for TPMT, suggesting its primary function may be in xenobiotic metabolism.
• TPMT is localized to the cytoplasm, as indicated by its subcellular location annotation.
• The protein undergoes post-translational modifications, including acetylation and phosphorylation, as identified through proteomics.
• TPMT belongs to the methyltransferase family and is characterized by keywords: 3D-structure, S-adenosyl-L-methionine, reference proteome, among others.

Experimental Guidance and Technical Tips

• The immunogen spans the full-length human TPMT protein (aa 1-245), suggesting the antibody may recognize multiple epitopes; therefore, it might be suitable for detecting denatured and native forms in WB and ELISA.
• For Western blot, the calculated molecular weight of TPMT is ~28 kDa; confirm actual band migration using appropriate control lysates.
• In immunofluorescence/ICC, expect a cytoplasmic localization pattern; optimize fixation and permeabilization conditions for your cell type.
• Cross-reactivity with mouse and rat is reported; verify species compatibility in your experimental system if using non-human models.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your trusted partner for IVD raw materials, providing this TPMT antibody alongside a comprehensive portfolio for drug metabolism and pharmacogenomics research. We support diagnostic manufacturers and labs with validated antibody pairs, bulk reagents, and customized sourcing solutions—streamlining your path from assay development to clinical application.