Anti-TPX2 Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - Q9ULW0

Core Product Specifications and Parameters

Biological Background: TPX2 Function and Localization

• TPX2 (Targeting Protein for Xklp2) is a pivotal spindle assembly factor required for normal mitotic spindle formation and microtubule nucleation during apoptosis. Related references: PMID:18663142 PMID:19208764 PMID:37728657
• It activates Aurora A kinase (AURKA) by promoting autophosphorylation at Thr-288 and protecting this residue from dephosphorylation, essential for spindle microtubule organization. Related references: PMID:18663142 PMID:19208764
• TPX2 activity is regulated by importin-alpha; at the onset of mitosis, GOLGA2 displaces importin-alpha, freeing TPX2 to activate AURKA and trigger local microtubule nucleation. Related references: PMID:26165940
• The protein is essential for chromatin- and kinetochore-dependent microtubule nucleation, contributing to accurate chromosome segregation. Related references: PMID:37728657
• Subcellularly, TPX2 localizes to the nucleus, cytoplasm, mitotic spindle, and spindle poles, consistent with its dynamic role during cell division.
• It is specifically expressed in lung carcinoma cell lines but absent in normal lung tissues, highlighting its association with cancer.
• Post-translational modifications include acetylation, phosphorylation, isopeptide bond formation, and ubiquitin-like conjugation, which may regulate its function.
• Alternative splicing generates multiple isoforms, expanding its functional diversity.

Experimental Guidance and Technical Tips

• This polyclonal antibody, raised against recombinant human TPX2 (aa 1-250), is suitable for detecting endogenous TPX2 in human and rat samples via WB, IHC-P, IF/ICC, and ELISA.
• For Western blotting, a band around 86 kDa is expected; optimize primary antibody dilution (e.g., 1:500-1:2000) based on sample and detection system.
• In IHC-P and IF/ICC, include appropriate controls and consider antigen retrieval methods to enhance signal.
• ELISA can be used for quantitative analysis, but pairing with a matched capture antibody may be necessary for sandwich ELISA.
• Validate the antibody's specificity in your experimental system, especially if using tissues with low TPX2 expression.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your reliable source for IVD raw materials, offering validated antibodies, antigen reagents, and technical support for diagnostic developers. This anti-TPX2 antibody supports research into cell cycle regulation, mitosis, and cancer diagnostics. Contact us for bulk procurement, custom antibody development, and integrated supply chain solutions tailored to your needs.