Thromboxane synthase Rabbit Polyclonal Antibody for WB, ELISA - P24557

Core Product Specifications and Parameters

Biological Background: Thromboxane synthase Function and Localization

• Thromboxane-A synthase (TBXAS1, CYP5A1, TXAS) is a cytochrome P450 family enzyme central to the arachidonic acid metabolic pathway.
• Catalyzes the conversion of prostaglandin H2 (PGH2) to thromboxane A2 (TXA2), a potent vasoconstrictor and platelet aggregation inducer. Simultaneously produces 12-hydroxyheptadecatrienoic acid (12-HHT) and malondialdehyde (MDA), which mediates DNA damage.
  Related references: PMID:11097184 PMID:11297515 PMID:22735388 PMID:9873013
• Displays dehydratase activity toward (15S)-hydroperoxy-(5Z,8Z,11Z,13E)-eicosatetraenoate (15(S)-HPETE), producing 15-KETE and 15-HETE.
  Related references: PMID:17459323
• Located primarily on the endoplasmic reticulum membrane, ensuring efficient coupling with upstream prostaglandin H synthases.
• Highly expressed in platelets; also found in lung, kidney, spleen, macrophages, and lung fibroblasts.
• Contains heme and iron cofactors and is classified as a monooxygenase, isomerase, and lyase. Multiple isoforms arise through alternative splicing.
• Genetic variants in TBXAS1 are associated with bleeding disorders such as Ghosal hematodiaphyseal dysplasia (BDPLT14), highlighting its role in hemostasis.

Experimental Guidance and Technical Tips

• For Western blotting (WB), a band around 61 kDa is anticipated in human and mouse platelet or lung tissue lysates. Use reducing conditions and validate with appropriate positive controls (e.g., platelet-rich plasma).
• The immunogen spans amino acids 355–534, corresponding to a C‑terminal region; therefore, the antibody may recognize full-length and certain truncated isoforms.
• In ELISA, the antibody can be used for antigen capture or detection; pairing with a second antibody against a distinct epitope is recommended for sandwich assays. Optimize concentrations in your sample matrix.
• Due to high target expression in platelets, avoid excessive freeze–thaw cycles of platelet samples to preserve epitope integrity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive portfolio of IVD raw materials, technical services, and consulting for diagnostic manufacturers and research laboratories. This thromboxane synthase antibody supports investigations into platelet biology, arachidonic acid metabolism, and hemostasis research, and can be paired with our ancillary reagents and custom bulk-sourcing solutions. Whether you need validated antibody pairs, bulk quantities, or rare-target sourcing, CamelBio serves as a reliable bridge from discovery to clinical application.