Anti-TRβ1/THRB Polyclonal Antibody for WB, ELISA - P10828

Core Product Specifications and Parameters

Biological Background: TRβ1/THRB Function and Localization

• Thyroid hormone receptor beta (TRβ1, encoded by THRB) is a nuclear receptor that mediates the physiological effects of thyroid hormones, including triiodothyronine (T3) and thyroxine (T4).
• As a member of the nuclear receptor subfamily 1 group A (NR1A2), TRβ1 is structurally homologous to the viral oncogene v-erbA and is classified as c-erbA-beta.
• The receptor functions as a ligand-dependent transcription factor, capable of both activating and repressing gene expression, thereby regulating metabolic rate, development, and homeostasis.
• TRβ1 localizes predominantly to the nucleus, where it binds to specific DNA sequences known as thyroid hormone response elements (TREs) in the promoter regions of target genes.
• Alternative splicing yields multiple isoforms, including TRβ1 and TRβ2, which differ in their N-terminal regions and show tissue-specific expression patterns.
• The protein contains a DNA-binding domain with two zinc-finger motifs and a ligand-binding domain that undergoes a conformational change upon hormone binding, enabling interaction with coactivators or corepressors.
• Mutations in THRB are linked to resistance to thyroid hormone (RTH) syndrome and can cause deafness, underscoring its essential role in auditory development.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N‑terminal 1‑263 amino acids of human TRβ1, which may include isoform-specific sequences; consider verifying specificity for TRβ1 versus TRβ2 using isoform-specific controls.
• For western blotting, a band near 53 kDa is expected for the full-length TRβ1 protein; additional bands may represent alternative isoforms, splice variants, or post‑translational modifications.
• Cross‑reactivity with mouse and rat has been reported; validate performance in your specific sample system and consider using species‑matched positive controls.
• This polyclonal format may offer increased sensitivity for detecting low‑abundance targets but should be carefully titrated to minimize background in ELISA and WB applications.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio equips diagnostic manufacturers, research labs, and IVD developers with reliable access to validated raw materials—including high‑quality polyclonal antibodies like this anti‑TRβ1/THRB antibody—to accelerate assay development from concept to clinic. Our portfolio covers nuclear receptor and transcription factor targets, supported by bulk ancillary reagents, optimized antibody pairs, and custom sourcing for rare targets. Whether you require one‑stop purchasing for routine supplies or tailored support for thyroid‑related biomarker projects, CamelBio bridges your research needs with dependable supply solutions.