Anti-TYMS Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - P04818

Core Product Specifications and Parameters

Biological Background: TYMS Function and Localization

• Thymidylate synthase (TYMS, also known as TS, TMS, DKCD, HST422) is a key enzyme in nucleotide metabolism, catalyzing the conversion of deoxyuridine monophosphate (dUMP) to deoxythymidine monophosphate (dTMP) using 5,10-methylenetetrahydrofolate as a methyl donor.
• This reaction is essential for the de novo synthesis of thymidylate, a critical precursor for DNA replication and repair, and occurs in both the nucleus and mitochondria.
• TYMS is localized to the nucleus, cytoplasm, and mitochondria, where it can be found associated with the mitochondrial inner membrane and matrix.
• Alternative splicing generates multiple isoforms, and the protein undergoes post-translational modifications including phosphorylation and ubiquitination.
• Mutations in TYMS are associated with diseases such as dyskeratosis congenita, a bone marrow failure syndrome.
• Functional annotations include methyltransferase, transferase, nucleotide biosynthesis, and disease variant.

Experimental Guidance and Technical Tips

• The immunogen spans the full-length human TYMS protein, making it suitable for detecting total thymidylate synthase in WB, IHC-P, and ELISA applications.
• For Western blotting, expect a band around 36 kDa; use human or mouse cell lysates with known TYMS expression as positive controls.
• In immunohistochemistry (IHC-P), optimize antigen retrieval and validate in proliferation-active tissues (e.g., tumor sections).
• For ELISA, the antibody can be employed as a capture or detection reagent; titrate to determine optimal working concentrations.
• Cross-reactivity is confirmed for human and mouse; if other species are needed, perform additional validation.

CamelBio: Your One-Stop Sourcing Bridge

This TYMS antibody supports research into nucleotide metabolism and cancer diagnostics. CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. Contact our team to discuss custom sourcing and technical support for your assay development needs.