Anti-PLS1 (Plastin-1) Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - Q14651

Core Product Specifications and Parameters

Biological Background: Plastin-1 Function and Localization

• Plastin-1 (I-plastin), encoded by the PLS1 gene, is an actin-bundling protein that belongs to the plastin family.
• In the inner ear, plastin-1 is required for stereocilia formation; it mediates liquid packing of actin filaments, which is essential for stereocilia to grow to their proper dimensions.
• Tissue expression is highest in the small intestine, colon, and kidney, with relatively lower levels in the lung and stomach.
• Subcellular localization is predominantly cytoplasmic and concentrated in cell projections, particularly stereocilia.
• The protein contains multiple actin-binding domains and calcium-binding EF-hand motifs, enabling regulated cross-linking of actin filaments.
• Post-translational modifications include acetylation and phosphorylation, which may modulate its actin-bundling activity.
• Variants in PLS1 are associated with autosomal dominant non-syndromic deafness (DFNA76), highlighting its critical role in auditory function.

Experimental Guidance and Technical Tips

• The immunogen comprises the N-terminal region (amino acids 1-110) of human PLS1, which is likely to be accessible in the native protein.
• For western blot, the predicted molecular weight is approximately 70 kDa; we recommend using appropriate positive controls such as intestinal or kidney tissue lysates.
• For immunohistochemistry (paraffin sections), tissues with high PLS1 expression (e.g., small intestine, colon) should yield robust staining; appropriate antigen retrieval and antibody dilution optimization are advised.
• The antibody shows cross-reactivity with mouse and rat, making it suitable for studies in rodent models of hearing loss or intestinal biology.
• As with all polyclonal antibodies, lot-to-lot consistency should be evaluated for critical quantitative assays.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your integrated partner for IVD raw material sourcing, offering validated antibody pairs, bulk ancillary reagents, and tailored procurement for rare targets like plastin-1. With a focus on diagnostic manufacturing and research applications, we bridge the gap from concept to clinic, ensuring reliable supply and technical support for your cytoskeletal and auditory function studies. Contact us to streamline your sourcing strategy and accelerate your project timelines.