Anti-THBS1 Polyclonal Antibody for IHC-P, ELISA - P07996

Core Product Specifications and Parameters

Biological Background: THBS1 Function and Localization

• Thrombospondin-1 (THBS1), also known as Glycoprotein G, is an adhesive glycoprotein that mediates cell-to-cell and cell-to-matrix interactions, playing a critical role in tissue architecture and cellular communication. Related references: PMID:15014436, PMID:18285447, PMID:2430973
• THBS1 is a multifunctional protein involved in key processes such as inflammation, angiogenesis, wound healing, reactive oxygen species (ROS) signaling, nitric oxide (NO) signaling, apoptosis, cellular senescence, and metabolic homeostasis. Related references: PMID:10613822, PMID:11134179, PMID:1371676
• It functions as a ligand for CD47, modulating NO signaling and contributing to blood pressure regulation, and also promotes endothelial cell senescence via CD47-mediated ROS production. Related references: PMID:19004835, PMID:8550562, PMID:29042481
• THBS1 interacts with CD36 to induce apoptosis in podocytes and suppress angiogenesis, highlighting its role in vascular biology and metabolic disorders. Related references: PMID:10613822, PMID:11134179, PMID:1371676
• Through binding to SIRPA, THBS1 stimulates NADPH oxidase-derived ROS production in non-phagocytic cells and may influence adipose inflammatory activity in obesity. Related references: PMID:24511121
• THBS1 is expressed by platelets and monocyte-derived dendritic cells, and it negatively modulates dendritic cell activation, contributing to immune homeostasis. Related references: PMID:101549, PMID:14568985
• Subcellular localization includes secretion into the extracellular space and matrix, as well as association with the endoplasmic reticulum and sarcoplasmic reticulum, consistent with its role in ER stress response and extracellular signaling.
• Keywords: calcium-binding, heparin-binding, EGF-like domains, and disulfide bonds contribute to its structural stability and interaction capabilities.

Experimental Guidance and Technical Tips

• For IHC-P, consider optimizing antigen retrieval conditions and using appropriate tissue controls to enhance signal specificity.
• Validate antibody specificity by peptide blocking assay, especially given the synthetic peptide immunogen spanning amino acids 150-250.
• For ELISA, ensure proper coating buffer and blocking conditions, and include a standard curve with known THBS1 concentrations.
• Cross-reactivity with mouse suggests potential for murine model studies; however, perform species-specific validation to confirm reactivity patterns.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers comprehensive IVD raw-material solutions, including validated antibody pairs, optimized polyclonal antibodies, and bulk ancillary reagents to support your diagnostic and research workflows. For studies on thrombospondin-1 and angiogenesis, we can assist in sourcing rare target raw materials and provide technical consulting from concept to clinic. Partner with us for reliable, integrated supply chain support.