TCHH Rabbit Polyclonal Antibody for WB and ELISA - Q07283

Core Product Specifications and Parameters

Biological Background: Trichohyalin Function and Localization

• Trichohyalin (TCHH) is a large intermediate filament-associated protein encoded by the TCHH gene, also known by aliases THH, THL, TRHY, and UHS3, with a calculated molecular weight of approximately 254 kDa.
• TCHH associates in regular arrays with keratin intermediate filaments (KIF) in the inner root sheath cells of the hair follicle and the granular layer of the epidermis, contributing to the structural integrity of these tissues.
• During terminal differentiation, TCHH becomes cross-linked to KIF via isodipeptide bonds, potentially acting as a scaffold protein in the organization of the cell envelope and anchoring it to the KIF network.
• TCHH may be involved in its own calcium-dependent postsynthetic processing, highlighting the importance of metal-binding and citrullination modifications in its maturation.
• The protein is predominantly expressed in hard keratinizing tissues, including the inner root sheath and medulla of hair follicles, as well as the filiform papillae of the dorsal tongue epithelium.
• Key biological processes associated with TCHH include keratinization, calcium binding, and proteolytic processing, as indicated by UniProt keywords.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 100–300 of human TCHH. For Western blot, expect a band near 254 kDa; use a high-molecular-weight protein ladder and consider extended transfer times.
• Validate antibody reactivity in relevant tissue samples such as skin or hair follicle extracts, which express TCHH endogenously.
• For ELISA, the recombinant immunogen or purified protein can be used as a coating antigen; perform checkerboard titrations to optimize conditions.
• As a polyclonal antibody, lot-to-lot consistency should be confirmed for critical quantitative assays.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with streamlined access to high-quality IVD raw materials like anti-TCHH antibodies for dermatology and keratinization studies. From validated antibody pairs to bulk ancillary reagents, our platform simplifies procurement for projects requiring rare or specialized targets. Partner with CamelBio to accelerate your assay development from concept to clinic.