Anti-TRH Polyclonal Antibody for WB, IF-P, ELISA - P20396

Core Product Specifications and Parameters

Biological Background: TRH Function and Localization

• Encoded by the TRH gene, pro-thyrotropin-releasing hormone (pro-TRH / prothyroliberin) is a precursor protein that undergoes proteolytic cleavage to generate the mature thyrotropin-releasing hormone tripeptide and other cryptic peptides.
• As a critical component of the hypothalamic-pituitary-thyroid (HPT) axis, TRH stimulates the secretion of thyroid-stimulating hormone (TSH) from the anterior pituitary, thereby regulating thyroid hormone synthesis.
• Beyond its endocrine role, TRH functions as a modulator of hair growth: it promotes hair-shaft elongation, prolongs the anagen (growth) phase, and antagonizes catagen (regression) induction by TGFB2.
• In hair follicle biology, TRH stimulates proliferation and inhibits apoptosis of hair matrix keratinocytes, highlighting its importance in skin and hair physiology.
• The protein is synthesized with a signal peptide and is secreted; mature TRH is released into the hypophyseal portal circulation or acts locally in peripheral tissues.
• Tissue expression is prominent in the hypothalamus, and at the protein level, TRH is detected in the hair follicle epithelium, consistent with its dual systemic and paracrine roles.
• UniProt keywords include “Amidation”, “Cleavage on pair of basic residues”, “Hormone”, “Secreted”, and “Repeat”, reflecting its biosynthetic processing and hormonal nature.

Experimental Guidance and Technical Tips

• The immunogen was designed using a synthetic peptide spanning amino acids 25–230 of human TRH (NP_009048.1), covering the pro‐region; this rabbit polyclonal antibody is expected to recognize both precursor and processed forms, although actual reactivity should be confirmed experimentally.
• For Western blotting, consider that the calculated molecular weight of full-length pro‐TRH is ~27 kDa, but tissue‐specific processing may yield bands of different sizes; validate in positive control lysates (e.g., hypothalamus, hair follicle) and include appropriate loading controls.
• In immunofluorescence (IF-P), this antibody may detect endogenous TRH in paraffin-embedded or frozen sections of hypothalamus or hair follicle; optimization of antigen retrieval and blocking is advised.
• For ELISA applications, the antibody can be used as a capture or detection reagent after verifying binding affinity and specificity with the target peptide or recombinant protein.
• Cross-reactivity is reported for human, mouse, and rat; when working with other species, perform a sequence alignment of the immunogen region and pilot experiments to assess suitability.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines the procurement of high-quality IVD raw materials, offering this TRH polyclonal antibody as part of a broader portfolio that includes validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents. As a one-stop sourcing partner for research and diagnostic development, CamelBio supports projects spanning the HPT axis, hair biology, and beyond, from initial screening to scale-up, with reliable access to rare target raw materials and technical consultation.