Anti-Tyrosine Hydroxylase Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - P07101

Core Product Specifications and Parameters

Biological Background: Tyrosine Hydroxylase (TH) Function and Localization

• Tyrosine hydroxylase (TH) catalyzes the rate-limiting step in catecholamine biosynthesis: the conversion of L-tyrosine to L-Dopa, a precursor for dopamine, noradrenaline, and adrenaline. This reaction uses tetrahydrobiopterin and molecular oxygen; it can also hydroxylate phenylalanine and tryptophan with lower specificity. Related references: PMID:15287903, PMID:1680128, PMID:17391063, PMID:24753243, PMID:34922205, PMID:8528210
• TH belongs to the biopterin-dependent aromatic amino acid hydroxylase family and requires iron as a cofactor.
• Alternative splicing produces multiple isoforms; some isoforms lack catalytic activity.
• Primarily expressed in the brain and adrenal glands, consistent with its role in neurotransmitter synthesis.
• Subcellular localization includes the cytoplasm (perinuclear region), nucleus, axon terminals, and synaptic vesicles.
• Phosphorylation regulates TH activity; it is identified as a phosphoprotein in proteomics studies.
• Disease-associated mutations in TH are linked to DYT5b dystonia, Parkinson's disease, and parkinsonism.

Experimental Guidance and Technical Tips

• For Western blot (WB), an expected band around 59 kDa may be detected; confirm using a positive control lysate from brain or adrenal tissue. Isoform-specific sizes may vary.
• In immunofluorescence (IF/ICC), permeabilization may be required to access cytoplasmic and nuclear pools of TH. Validate staining in relevant cell lines (e.g., SH-SY5Y neuroblastoma) or primary neurons.
• Since the immunogen is a synthetic peptide, blocking with the immunizing peptide can help confirm antibody specificity.
• For ELISA, optimize coating and detection conditions empirically. The antibody detects human, mouse, and rat TH, enabling cross-species assay development.
• Cross-reactivity with mouse and rat has been validated; still, appropriate controls should be included for new model systems.

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CamelBio supports diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials—from concept to clinic. Our portfolio includes validated antibody pairs, optimized monoclonal/polyclonal antibodies like this anti-Tyrosine Hydroxylase antibody, bulk ancillary reagents, and sourcing support for rare targets. Partner with us to streamline your catecholamine and neurodegeneration assay development.