Anti-TBL1X Polyclonal Antibody for WB and ELISA - O60907

Core Product Specifications and Parameters

Biological Background: TBL1X Function and Localization

• TBL1X (Transducin beta-like protein 1X, also known as SMAP55, EBI, TBL1) is an F-box-like/WD repeat-containing protein encoded by the TBL1X gene on chromosome X. It belongs to the WD repeat protein family and functions as an adaptor in transcriptional regulation.
• As an F-box-like protein, TBL1X is crucial for recruiting the ubiquitin/19S proteasome complex to nuclear receptor-regulated transcription units, enabling proteasome-dependent exchange of corepressor for coactivator complexes. Related references: PMID:14980219 PMID:21240272
• It is an integral component of nuclear receptor corepressor (NCoR) and silencing mediator for retinoid and thyroid receptors (SMRT) complexes, facilitating ligand-dependent transcriptional activation by nuclear receptors such as thyroid hormone receptor and retinoic acid receptor.
• TBL1X localizes predominantly to the nucleus, consistent with its role in transcriptional regulation.
• The protein is subject to post-translational modifications including acetylation and phosphorylation, and is targeted by ubiquitin and ubiquitin-like modifications, regulating its stability and activity.
• TBL1X is ubiquitously expressed across human tissues, underscoring its fundamental role in transcription.
• Mutations in TBL1X are associated with X-linked congenital hypothyroidism and deafness, highlighting its physiological importance in endocrine and auditory development.

Experimental Guidance and Technical Tips

• The immunogen corresponds to an internal sequence (aa 100-200) of human TBL1X; consider using this antibody in Western blotting to detect endogenous TBL1X at approximately 62 kDa in human, mouse, or rat nuclear extracts.
• For ELISA, validate coating conditions and antibody specificity using recombinant TBL1X protein or the immunizing peptide as a control.
• Optimize blocking and wash steps to reduce background, especially given the rabbit polyclonal format.
• Since TBL1X undergoes post-translational modifications, consider treating lysates with phosphatase inhibitors or performing immunoprecipitation to assess modified forms.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio partners with diagnostic manufacturers and research labs to streamline IVD raw material procurement. This anti-TBL1X polyclonal antibody supports transcriptional regulation and nuclear receptor signaling research. CamelBio offers a comprehensive portfolio including validated antibody pairs, monoclonal/polyclonal antibodies, and bulk ancillary reagents, with sourcing capabilities for rare targets to accelerate your assay development.