Anti-TRA2B Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - P62995

Core Product Specifications and Parameters

Biological Background: TRA2B Function and Localization

• TRA2B (Transformer-2 protein homolog beta), also known as SFRS10 or SRFS10, is a member of the serine/arginine-rich (SR) family of splicing regulators, essential for alternative pre-mRNA splicing.
• It functions as a sequence-specific RNA-binding protein that can either activate or repress exon inclusion, modulating the splicing of numerous genes critical for cellular processes.
• TRA2B plays a key role in the splicing of the survival motor neuron SMN2 pre-mRNA, promoting exon 7 inclusion additively with RBMX, which is crucial for motor neuron survival.
• It also activates the splicing of MAPT/Tau exon 10, contributing to tau isoform balance in neuronal cells.
• Subcellularly, TRA2B localizes to the nucleus, consistent with its role in pre-mRNA processing.
• The protein undergoes multiple post-translational modifications, including phosphorylation, acetylation, methylation, and ubiquitination, which likely regulate its activity and stability.
• Tissue expression is highest in heart, skeletal muscle, and pancreas, with lower levels in kidney, brain, and placenta, suggesting specialized roles in these tissues.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide spanning the N-terminal region (amino acids 1-100) of human TRA2B, designed to target the endogenous protein across applications.
• For Western blot (WB), TRA2B is predicted to migrate at approximately 34 kDa; use appropriate molecular weight markers and consider testing a range of lysate inputs.
• In immunofluorescence/immunocytochemistry (IF/ICC), note the nuclear localization of TRA2B; optimize fixation/permeabilization conditions for nuclear antigen detection.
• This antibody shows cross-reactivity with human, mouse, and rat, allowing multi-species studies; validate in the relevant sample system before quantitative assays.
• For ELISA, consider using this antibody as a capture or detection reagent when paired with another clone or format; optimize coating/buffer conditions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research labs by providing a comprehensive portfolio of IVD raw materials, including validated antibodies like this anti-TRA2B rabbit polyclonal antibody for splicing research and assay development. With capabilities ranging from antibody pair screening to bulk ancillary reagent supply, CamelBio simplifies the sourcing of rare targets and custom-formulated reagents for clinical and translational workflows.