Anti-WT1 Polyclonal Antibody for WB, IHC-P, ELISA - P19544

Core Product Specifications and Parameters

Biological Background: WT1 Function and Localization

• WT1 binds to the DNA sequence 5'-GCG(T/G)GGGCG-3' to regulate target gene expression, including EPO, and is essential for urogenital system development. Related references: PMID:7862533 PMID:17716689 PMID:25258363
• The protein exhibits both tumor suppressor and oncogenic functions depending on cellular context.
• WT1 has multiple isoforms with distinct activities. Isoforms lacking the KTS motif function as transcription factors, while those containing KTS may bind mRNA and participate in RNA processing or splicing. Related references: PMID:15520190 PMID:16934801
• Isoform 1 has lower DNA-binding affinity and can bind RNA. Related references: PMID:19123921
• WT1 is predominantly localized in the nucleus, including the nucleolus, nucleoplasm, and nuclear speckles, but can also be found in the cytoplasm.
• It is expressed in the kidney and a subset of hematopoietic cells.
• The protein contains zinc-finger domains and participates in RNA editing, alternative splicing, and ubl conjugation, consistent with its roles in transcription and RNA metabolism.

Experimental Guidance and Technical Tips

• For Western blot (WB), the expected molecular weight is approximately 49 kDa. Consider including protease inhibitors to prevent degradation.
• The immunogen corresponds to the N-terminal region (aa 1-302), present in multiple WT1 isoforms. This antibody may detect various isoforms; validate band patterns using relevant cell lines or tissues.
• For IHC-P, optimize antigen retrieval and include appropriate tissue controls (e.g., kidney or WT1-positive tumors).
• In ELISA, use the antibody as a capture or detection reagent following standard protocols; confirm specificity with recombinant WT1 protein.
• Cross-reactivity with mouse and rat suggests potential utility in rodent models; always validate in the specific experimental system.
• Avoid repeated freeze-thaw cycles to maintain antibody integrity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers and research institutes a one-stop platform for IVD raw materials, technical services, and consulting—from early development to clinical readiness. This WT1 polyclonal antibody supports studies in urogenital development and cancer biology. Explore our portfolio of validated antibody pairs, monoclonal antibodies, and bulk ancillary reagents to accelerate your assay development.