Anti-TSHB Rabbit Polyclonal Antibody for WB, ELISA - P01222

Core Product Specifications and Parameters

Biological Background: TSHB Function and Localization

• TSHB encodes thyrotropin subunit beta (TSH-beta), the hormone-specific subunit of thyroid-stimulating hormone (TSH), which pairs with the common alpha subunit (CGA) to form the active heterodimeric glycoprotein hormone.
• TSH is indispensable for the control of thyroid structure and metabolism, stimulating the thyroid gland to produce and release thyroid hormones that regulate growth, development, and energy expenditure.
• The TSHB protein is secreted and functions as a hormone within the hypothalamic-pituitary-thyroid axis.
• Structural integrity is maintained by several disulfide bonds, and the protein undergoes glycosylation, critical for proper folding, stability, and receptor interaction.
• Alternative splicing of the TSHB pre-mRNA generates multiple transcript variants, though the functional significance of these isoforms requires further study.
• Mutations in the TSHB gene are associated with congenital hypothyroidism, a disease variant leading to inadequate thyroid hormone production from birth.
• The protein has been extensively characterized by approaches including direct protein sequencing and proteomics, and contains a signal peptide for secretion.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide from the C-terminal region (amino acids 50 to the C-terminus), suggesting that the antibody may recognize the mature TSH beta chain; verification in relevant sample systems (e.g., pituitary or serum) is recommended.
• For Western blot, consider using reducing conditions to resolve possible disulfide-linked oligomers, and validate the observed band against the predicted 16 kDa molecular weight.
• This polyclonal antibody may detect multiple epitopes, potentially enhancing sensitivity in ELISA applications; appropriate controls and optimization of coating/detection conditions are advised.
• Cross-reactivity is reported for human, mouse, and rat, enabling translational studies; however, species-specific validation is recommended due to potential sequence variations.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines IVD raw-material procurement by offering a comprehensive portfolio that includes validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents. This TSHB antibody supports thyroid function research and diagnostic assay development. Our team can also source rare-target raw materials and provide technical consulting to help you move from concept to clinic efficiently.