Anti-sestrin 1 (PA26/SEST1) Rabbit Monoclonal Antibody for WB, IHC-P, ELISA - Q9Y6P5

Core Product Specifications and Parameters

Biological Background: Sestrin-1 Function and Localization

• Sestrin-1 (PA26/SEST1) is a stress-inducible protein encoded by the SESN1 gene, with multiple isoforms produced by alternative splicing.
• Functions as an intracellular leucine sensor that negatively regulates the mechanistic target of rapamycin complex 1 (TORC1) signaling pathway. In leucine absence, it binds the GATOR2 subcomplex and inhibits TORC1; leucine binding disrupts this interaction, activating TORC1. Related references: PMID:25263562, PMID:26449471
• Plays a role in oxidative stress defense by positively regulating NFE2L2-dependent transcription of antioxidant genes through SQSTM1-mediated autophagic degradation of KEAP1. Related references: PMID:23274085
• May possess alkylhydroperoxide reductase activity via its N-terminal domain, contributing to the prevention of reactive oxygen species (ROS) accumulation (By similarity).
• Was originally reported to contribute to oxidative stress resistance by reducing peroxiredoxin-1 (PRDX1), though later studies could not confirm this finding. Related references: PMID:15105503
• Localizes to both the nucleus and cytoplasm, consistent with its roles in signaling and stress responses.
• Widely expressed across tissues, underscoring its fundamental role in cellular homeostasis.
• Identified as a phosphoprotein, suggesting regulation by post-translational modification.

Experimental Guidance and Technical Tips

• The antibody was raised against a synthetic peptide spanning amino acids 100–200 of human sestrin-1; epitope specificity lies within this region. Consider sequence alignment when testing cross-reactivity with other species or isoforms.
• For Western blotting (WB), a predominant band at ~57 kDa is expected. Include appropriate positive and negative cell lysate controls to confirm detection specificity.
• In immunohistochemistry (IHC-P), optimize antigen retrieval conditions and primary antibody dilution to achieve robust staining. Nuclear and/or cytoplasmic signals may be observed.
• For ELISA, ensure the coating antigen or peptide matches the immunogen sequence to maintain assay sensitivity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers, contract research organizations, and academic laboratories by providing comprehensive, one-stop access to IVD raw materials. For studies on TORC1 signaling, oxidative stress, or metabolic regulation, we offer this high-quality anti-sestrin-1 monoclonal antibody along with validated antibody pairs, bulk ancillary reagents, and custom sourcing for rare or difficult targets. Let CamelBio be your procurement bridge from discovery to diagnostic development.