Anti-STIM1 Rabbit Monoclonal Antibody for WB, IHC-P, IP, ELISA - Q13586

Core Product Specifications and Parameters

Biological Background: STIM1 Function and Localization

• STIM1 is a Ca2+ sensor that gates CRAC and ARC channels at the plasma membrane, mediating store-operated Ca2+ entry (SOCE) in response to ER calcium store depletion (PubMed:15866891, PubMed:16005298, PubMed:16208375). Related references: PMID:15866891, PMID:16005298, PMID:16208375
• Upon ER calcium depletion, STIM1 translocates to ER-plasma membrane junctions and activates ORAI1, ORAI2, and ORAI3 channel subunits to drive sustained Ca2+ influx (PubMed:16208375, PubMed:16537481, PubMed:32415068). Related references: PMID:16208375, PMID:16537481, PMID:32415068
• STIM1 is involved in enamel formation and regulates type I interferon response by retaining the STING adapter at the endoplasmic reticulum (PubMed:24621671, PubMed:30643259). Related references: PMID:24621671, PMID:30643259
• The protein undergoes post-translational modifications, including glycosylation and phosphorylation, and contains a signal peptide and transmembrane domains.
• It is ubiquitously expressed in human primary cells and tumor cell lines, and localizes to the cell membrane, endoplasmic reticulum, cytoskeleton, and sarcoplasmic reticulum.
• Alternative splicing produces multiple isoforms; disease-associated variants are linked to immunodeficiency syndromes such as IMD10.

Experimental Guidance and Technical Tips

• The immunogen (amino acids 530–685 of human STIM1) corresponds to a cytoplasmic domain, likely detecting both full-length and truncated STIM1 by Western blot; validate sample preparation to preserve membrane protein integrity.
• For IHC-P, consider antigen retrieval conditions and validate staining specificity in human tissues with known STIM1 expression, using appropriate controls.
• For IP, the antibody may capture native STIM1 complexes; optimize lysis conditions and antibody concentration to maintain protein-protein interactions.
• ELISA performance should be calibrated with recombinant STIM1 protein as a standard; titrate antibody and detection reagents for optimal signal-to-noise ratio.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive partner for IVD raw material sourcing, providing validated monoclonal and polyclonal antibodies, antibody pairs, and bulk ancillary reagents. For calcium signaling and store-operated Ca2+ entry research, CamelBio supplies optimized anti-STIM1 antibodies like this recombinant rabbit monoclonal, alongside rare-target raw materials to support diagnostic development from early-stage research to clinical application.