Anti-Calsequestrin 1 Monoclonal Antibody for WB, IF-P, ELISA - P31415

Core Product Specifications and Parameters

Biological Background: Calsequestrin-1 Function and Localization

• Calsequestrin-1 (CASQ1), also known as calmitine or skeletal muscle isoform, is a high-capacity, moderate-affinity calcium-binding protein predominantly expressed in skeletal muscle.
• It functions as an internal calcium store within the sarcoplasmic reticulum, binding around 80 Ca2+ ions via clusters of acidic residues at the protein surface. Related references: PMID:28895244
• CASQ1 regulates calcium release through the ryanodine receptor RYR1, playing a critical role in triggering muscle contraction.
• It also negatively regulates store-operated calcium entry (SOCE). Related references: PMID:27185316
• Subcellular localization includes sarcoplasmic reticulum (lumen and membrane), endoplasmic reticulum, and mitochondrial matrix.
• The protein is expressed in myoblasts and is subject to post-translational modifications such as phosphorylation and glycosylation.
• Keywords: Calcium-binding, muscle protein, sarcoplasmic reticulum, signal.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 100–200 of human CASQ1; consider verifying the epitope in target species to ensure optimal binding.
• For western blot (WB), the observed band may appear around 45 kDa; validate with appropriate positive controls (e.g., skeletal muscle lysates).
• In immunofluorescence (IF), expect sarcoplasmic reticulum staining; permeabilization and fixation conditions should be optimized for muscle tissue.
• ELISA applications may require coating with recombinant or native protein; use a validated detection system to confirm specificity.
• Cross-reactivity with mouse and rat CASQ1 has been reported; verify in the relevant sample system if species-specific applications are critical.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. This anti-Calsequestrin 1 monoclonal antibody supports research in muscle physiology and calcium signaling; we offer validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents. For rare targets or custom sourcing needs, CamelBio bridges the gap between discovery and commercial application, ensuring reliable supply for your diagnostic development.