SCRN1 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - Q12765

Core Product Specifications and Parameters

Biological Background: Secernin-1 Function and Localization

• Secernin-1 (SCRN1), also known as SES1, is a protein encoded by the SCRN1 gene in humans.
• It plays a role in regulating exocytosis in mast cells, enhancing both the extent of secretion and the sensitivity to calcium stimulation.
• SCRN1 localizes predominantly to the cytoplasm, as indicated by subcellular localization data.
• The protein undergoes alternative splicing, producing multiple isoforms.
• Post-translational modifications include acetylation, as noted in proteomic studies.
• It has been identified through direct protein sequencing and proteomics identification, confirming its expression in reference proteomes.
• Further functional studies are needed to fully elucidate its role in human physiology, though current evidence suggests involvement in secretory pathways.

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 195-414 of human SCRN1, covering the C-terminal region, which may contribute to epitope specificity.
• For Western blot, the expected band is around 46 kDa; use standard protein extraction and loading protocols, and consider optimizing primary antibody dilution based on sample type and expression levels.
• In IF/ICC, fixation and permeabilization conditions should be empirically determined to preserve target epitopes and cellular morphology.
• Cross-reactivity has been reported for human, mouse, and rat; validate species reactivity in the relevant sample system before large-scale experiments.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and researchers with reliable, validated antibodies for secretory pathway targets like SCRN1, supporting exocytosis and mast cell biology studies. Our portfolio includes optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing, ensuring a continuous supply chain from concept to clinic. Partner with CamelBio for comprehensive IVD raw material solutions and accelerate your next breakthrough.