Anti-CD66a/CEACAM1 Rabbit Monoclonal Antibody for WB, IF-P, IHC-P, FC, ELISA - P31809

Core Product Specifications and Parameters

Biological Background: CD66a/CEACAM1 Function and Localization

• Mediates calcium-independent homophilic cell adhesion, facilitating cell-cell interactions and tissue architecture. Related references: PMID:1633107
• Functions as a coinhibitory receptor in T-cells and NK cells, suppressing TCR-mediated cytotoxicity and cytokine production through PTPN6 recruitment and dephosphorylation of CD247 and ZAP70. Related references: PMID:17081782, PMID:22496641
• Negatively regulates neutrophil activity by inhibiting IL1B production and downregulating CSF3R-STAT3 signaling via PTPN6. Related references: PMID:21029969, PMID:22496641
• Acts as a receptor for murine coronavirus (MHV), mediating viral entry through interaction with the S1 spike glycoprotein.
• Serves as an angiogenic activator, promoting endothelial cell differentiation, migration, and arteriogenesis post-ischemia. Related references: PMID:16680193, PMID:22962327
• Localizes to the lateral, apical, and basal cell membranes, adherens junctions, and microvilli, consistent with its adhesion and signaling roles.
• Expressed in granulocytes, lymphocytes, liver, bone, and intestinal T-cells, with different isoforms preferentially expressed in distinct tissues. Related references: PMID:11994468, PMID:21029969, PMID:23123061, PMID:25490771, PMID:25908210
• Multiple isoforms arise from alternative splicing, with predicted molecular weights of 57, 50, 37, and 30 kDa, all recognized by this antibody.

Experimental Guidance and Technical Tips

• The immunogen encompasses the N-terminal Ig-like domains (aa 35–428), which are conserved between mouse and rat, supporting observed cross-reactivity. For rat samples, verify expression in target tissue as some rat strains may express lower levels.
• For western blotting, multiple bands corresponding to the listed isoforms may be detected; consider using reducing conditions and confirm identity with appropriate controls.
• In immunohistochemistry (IHC-P), antigen retrieval may enhance staining, and isotype-matched negative controls are recommended to assess specificity.
• For flow cytometry, live-cell surface staining is feasible without permeabilization, as CEACAM1 is a transmembrane protein. Titrate the antibody to determine optimal concentration for your cell model.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research institutes with a comprehensive portfolio of IVD raw materials, including validated antibody pairs and optimized monoclonal antibodies like this anti-CD66a/CEACAM1 clone. We streamline procurement from concept to clinic, offering bulk ancillary reagents and rare-target sourcing for immunology and infectious disease assay development. Let CamelBio be your partner in accelerating reliable diagnostic solutions.