Anti-Alpha-1 Antitrypsin (SERPINA1) Monoclonal Antibody for WB, IF/ICC, ELISA - P01009

Core Product Specifications and Parameters

Biological Background: Alpha-1 Antitrypsin Function and Localization

• Alpha-1-antitrypsin (SERPINA1), also known as alpha-1 protease inhibitor or serpin A1, is a 46.7 kDa secreted glycoprotein and a member of the serpin superfamily.
• It functions primarily as an inhibitor of serine proteases, with elastase as its main target, and also inhibits trypsin, chymotrypsin, plasmin, thrombin, and plasminogen activator.
• The aberrant form of SERPINA1 inhibits insulin-induced NO synthesis in platelets, decreases coagulation time, and exhibits proteolytic activity against insulin and plasmin.
• A reversible chymotrypsin inhibitor isoform exists that inhibits elastase but not trypsin, and its major physiological role is protection of the lower respiratory tract against proteolytic destruction by human leukocyte elastase (HLE).
• SERPINA1 is localized to the secretory pathway, including the endoplasmic reticulum, and is secreted into the extracellular space and extracellular matrix.
• It is ubiquitously expressed, with known expression in leukocytes and plasma.
• Post-translational modifications include glycosylation and phosphorylation; it is classified as an acute phase protein and plays roles in blood coagulation and hemostasis.

Experimental Guidance and Technical Tips

• The recombinant immunogen covers amino acids 25–418, corresponding to the mature secreted form; consider this when detecting intracellular precursor forms.
• For Western blot, a band around 47 kDa (predicted) may be observed, but glycosylation can increase the apparent molecular weight.
• Applications validated include WB, IF/ICC, and ELISA; verify optimal dilutions in your specific experimental system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers Alpha-1 Antitrypsin (SERPINA1) antibodies as part of a comprehensive portfolio of IVD raw materials for protease inhibitor and respiratory disease research. With validated antibody pairs, bulk monoclonal and polyclonal antibodies, and ancillary reagents, we support diagnostic manufacturers from concept to clinic. Inquire about custom sourcing for rare targets and tailored assay development needs.