Anti-Sestrin 2 Rabbit Monoclonal Antibody for WB, ELISA - P58004

Core Product Specifications and Parameters

Biological Background: Sestrin 2 Function and Localization

• Sestrin 2 (SESN2), also known as Hypoxia-induced gene HI95, is a stress-inducible metabolic regulator that acts as an intracellular leucine sensor and a crucial modulator of the mTORC1 signaling pathway.
• It functions as an intracellular leucine sensor that negatively regulates mTORC1 signaling via the GATOR complex; in the absence of leucine, Sestrin 2 binds GATOR2 and prevents mTORC1 activation. Related references: PMID:18692468, PMID:25263562, PMID:25457612
• Direct binding of leucine to SESN2 disrupts its interaction with GATOR2, thereby activating the TORC1 signaling pathway and promoting cellular anabolism. Related references: PMID:26449471, PMID:26586190, PMID:35114100
• May negatively regulate protein translation in response to endoplasmic reticulum stress by inhibiting mTORC1 signaling, thus serving as a key mediator of the unfolded protein response. Related references: PMID:24947615
• Positively regulates the transcription of genes involved in the oxidative stress response by NFE2L2, through facilitating SQSTM1-mediated autophagic degradation of KEAP1. Related references: PMID:23274085
• Possesses an N-terminal alkylhydroperoxide reductase domain that may prevent accumulation of reactive oxygen species; earlier reports of PRDX1 reduction could not be confirmed. Related references: PMID:26612684, PMID:15105503, PMID:19113821
• Predominantly localized to the cytoplasm, where it interacts with GATOR2 and other signaling components.
• Subject to post-translational modifications including phosphorylation, acetylation, and ubiquitin-like conjugation, and is widely expressed across human tissues.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a conserved central region (amino acids 300-400) of human Sestrin 2; consider using pre-immune serum or blocking peptides for specificity controls in critical experiments.
• For western blotting, a specific band is expected around 54 kDa; note that stress-induced modifications or sample preparation may cause slight shifts in migration; validate using positive and negative controls.
• This rabbit monoclonal antibody offers high lot-to-lot consistency and recognizes Sestrin 2 from human, mouse, and rat, making it suitable for cross-species comparative studies.
• Sample treatment known to induce Sestrin 2 expression (e.g., leucine deprivation, oxidative stress) may enhance detection; optimize cell culture conditions and lysis buffers for your experimental system.

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CamelBio provides diagnostic manufacturers and researchers with comprehensive access to IVD raw materials, including this high-quality anti-Sestrin 2 monoclonal antibody. Our portfolio includes validated antibody pairs, optimized monoclonal and polyclonal antibodies, and ancillary reagents for immunoassay development. For specialized targets or bulk requirements, our sourcing network ensures reliable supply from R&D to clinical application.