Anti-Stathmin 1 Rabbit Polyclonal Antibody for WB and ELISA - P16949

Core Product Specifications and Parameters

Biological Background: Stathmin 1 Function and Localization

• Stathmin 1 (STMN1), also known as oncoprotein 18 (Op18), phosphoprotein p19, metablastin, and prosolin, is a key regulator of microtubule dynamics.
• It destabilizes microtubules by preventing assembly and promoting disassembly, thereby controlling the microtubule filament system.
• Phosphorylation at Ser-16 is implicated in axon formation during neurogenesis, as suggested by similarity studies.
• The protein is involved in the control of learned and innate fear behaviors (by similarity).
• Stathmin 1 is predominantly cytosolic and associates with the cytoskeleton.
• It is ubiquitously expressed, with highest levels in fetal and adult brain, spinal cord, and cerebellum; lower in adult liver; and notably elevated in acute leukemia cells.
• Post-translational modifications include acetylation, phosphorylation, and methylation, which modulate its activity and interactions.
• Alternative splicing generates multiple isoforms, contributing to functional diversity.

Experimental Guidance and Technical Tips

• The immunogen comprises a recombinant fusion protein spanning amino acids 1-149 of human Stathmin 1. When designing similar reagents, consider this N-terminal region for potential epitope coverage.
• For Western blot, the expected molecular weight of Stathmin 1 is approximately 17 kDa. Validate the detection in relevant cell lysates.
• The antibody cross-reacts with mouse Stathmin 1; perform species-specific validation if using mouse models.
• Optimization of dilution factors and blocking conditions is recommended for both WB and ELISA to minimize background.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive portfolio of IVD raw materials, including validated antibody pairs and optimized polyclonal antibodies like this anti-Stathmin 1 reagent. Whether you require bulk ancillary reagents, rare target antibodies, or technical consultation for microtubule-related cancer or neuroscience research, our one-stop supply chain bridges your concept to clinical application.