Anti-Sec61A Rabbit Monoclonal Antibody for WB, ELISA - Q9H9S3

Core Product Specifications and Parameters

Biological Background: Sec61A Function and Localization

• Sec61A (SEC61A2) encodes the alpha isoform 2 subunit of the Sec61 translocon complex, a central component of the protein translocation machinery in the endoplasmic reticulum (ER).
• The Sec61 complex forms a transmembrane channel that facilitates the cotranslational translocation of signal peptide-bearing nascent polypeptides across the ER membrane.
• It serves a dual function as both a ribosome receptor and a gated pore, thereby coupling translation elongation with polypeptide transport into the ER lumen.
• This protein is an integral multi-pass membrane protein localized to the endoplasmic reticulum membrane.
• Alternative splicing generates multiple transcript variants, which may contribute to functional diversity and tissue-specific regulation of ER protein import.
• The protein is classified under ER membrane transport proteins and plays a key role in the secretory pathway, protein quality control, and cellular homeostasis.
• Post-translational modifications and regulatory interactions modulate its activity, though detailed mechanisms remain under investigation.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the C-terminal region (amino acids 350-450) of human Sec61A; therefore, the antibody likely recognizes an epitope within this cytoplasmic domain. Consider using C-terminal tags or truncations for epitope mapping.
• For Western blot, a band near 52 kDa is expected. Include positive controls such as HeLa, HEK293, or liver tissue lysates, as Sec61A is ubiquitously expressed.
• When performing ELISA, the synthetic peptide immunogen can serve as a positive coating antigen. Optimize the peptide coating concentration and antibody dilution in pilot experiments.
• Validate cross-reactivity in mouse and rat samples using tissue-derived lysates, as sequence homology may influence detection efficiency.
• For immunoprecipitation or IF applications, empirical testing is recommended since these are not listed in the validated applications.

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