Anti-STAT6 Rabbit Monoclonal Antibody for WB, IF/ICC, ELISA - P42226

Core Product Specifications and Parameters

Biological Background: STAT6 Function and Localization

• STAT6 is a member of the signal transducer and activator of transcription (STAT) family, functioning as both a signal transducer and transcriptional activator.
• It mediates cellular responses to IL-4 and IL-3, playing a pivotal role in cytokine signaling pathways.
• Multiple isoforms exist due to alternative splicing, including STAT6B and STAT6C, which may have distinct regulatory functions.
• Upon activation, STAT6 translocates from the cytoplasm to the nucleus, where it binds DNA to regulate gene expression.
• The protein contains an SH2 domain critical for interaction with phosphorylated receptors and dimerization.
• Post-translational modifications such as phosphorylation, acetylation, and ADP-ribosylation modulate its activity and stability.
• Associated with disease variants, suggesting its implication in human pathologies.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a C-terminal sequence (aa 747-847) of human STAT6, which is conserved in mouse, supporting the observed cross-reactivity.
• For Western blotting, expect a band near 94 kDa; validate specificity using knockout or knockdown controls if possible.
• In immunofluorescence/ICC, consider including IL-4 stimulation to observe nuclear translocation.
• The monoclonal format ensures high specificity and reproducibility; however, we recommend titrating the antibody for each application and sample type.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio empowers diagnostic manufacturers and researchers with comprehensive access to validated IVD raw materials. Our portfolio includes monoclonal and polyclonal antibodies, antibody pairs, and ancillary reagents for STAT6 and related cytokine signaling targets, supporting your assay development from concept to market. Leverage our expertise in rare target sourcing to accelerate your STAT6-related projects.