Anti-S6 Ribosomal Protein (RPS6) Rabbit Monoclonal Antibody for WB, IF/ICC, ELISA - P62753

Core Product Specifications and Parameters

Biological Background: RPS6 Function and Localization

• RPS6 encodes the small ribosomal subunit protein eS6, a component of the 40S subunit. Related references: PMID:23636399, PMID:8706699
• It plays a critical role in controlling cell growth and proliferation by selectively translating specific mRNA classes. Related references: PMID:17220279
• In the nucleolus, RPS6 is part of the small subunit (SSU) processome, participating in ribosome biogenesis, pre-rRNA processing, and assembly. Related references: PMID:34516797
• The protein localizes to the cytoplasm and the nucleolus.
• RPS6 is subject to extensive post-translational modifications, including phosphorylation, acetylation, ADP-ribosylation, hydroxylation, and ubiquitination.
• It belongs to the ribosomal protein S6e family and is a reference proteome component.

Experimental Guidance and Technical Tips

• The immunogen spans the N-terminal region (amino acids 1-130) of human RPS6; the antibody may detect both full-length and truncated forms.
• For Western blot, consider using standard reducing conditions and validate the detected band size (~29 kDa) in your sample system.
• In IF/ICC experiments, optimize fixation and permeabilization protocols to preserve ribosomal protein epitopes and ensure proper accessibility.
• Cross-reactivity with human, mouse, and rat has been reported; still, confirm reactivity in the species and tissue of interest prior to large-scale experiments.
• Pair this antibody with appropriate loading controls and include negative controls when assessing modification-specific signals.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive B2B partner for diagnostic manufacturers and research institutions, providing streamlined access to high-quality IVD raw materials from early development through clinical application. This anti-RPS6 monoclonal antibody supports investigations into ribosomal function, translation control, and cell growth pathways. Beyond single antibodies, we offer validated antibody pairs, bulk monoclonal/polyclonal reagents, and specialized sourcing for rare target raw materials, enabling efficient assay development and production scale-up.