Anti-SSTR5 Monoclonal Antibody for WB, IHC-P, ELISA - P35346

Core Product Specifications and Parameters

Biological Background: SSTR5 Function and Localization

• SSTR5 (Somatostatin receptor type 5) is a G protein-coupled receptor that binds somatostatin-28 with high affinity and somatostatin-14 with lower affinity.
• Upon ligand binding, the receptor activates Gi/Go proteins to inhibit adenylyl cyclase, reducing intracellular cAMP levels.
• SSTR5 heterodimerizes with SSTR2, enhancing SSTR2-mediated cell growth inhibition.
• It is expressed at the cell membrane and is subject to post-translational modifications including glycosylation, palmitoylation, and phosphorylation (as indicated by keywords).
• Tissue expression includes the adult pituitary gland, heart, small intestine, adrenal gland, cerebellum, and fetal hypothalamus; it is absent in many fetal and adult peripheral organs.
• The protein contains seven transmembrane helices, characteristic of GPCRs, and a disulfide bond important for structural stability.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (aa 1-100) of human SSTR5; therefore, the antibody may recognize epitopes within this extracellular domain.
• For Western blotting, the predicted molecular weight of SSTR5 is approximately 39 kDa (consistent with the provided 39kDa), but post-translational modifications may cause minor shifts; consider using a positive control lysate from pituitary or heart tissue.
• In immunohistochemistry (paraffin-embedded sections), antigen retrieval with citrate buffer pH 6.0 is suggested; test the antibody on known SSTR5-expressing tissues (e.g., pituitary gland) to verify staining specificity.
• For ELISA, coating the synthetic peptide or recombinant protein fragment as capture antigen may be suitable; optimize the antibody concentration based on titration experiments.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive one-stop solution for IVD raw material sourcing, supporting somatostatin receptor research with high-quality antibodies like this SSTR5 Rabbit mAb. Our portfolio includes validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and customized sourcing for rare targets, streamlining your diagnostic development workflow from concept to clinic.