STX1A Rabbit Monoclonal Antibody for WB, IF-P, IHC-P, ELISA - Q16623

Core Product Specifications and Parameters

Biological Background: Syntaxin-1A Function and Localization

• Syntaxin-1A, also known as neuron-specific antigen HPC-1, is encoded by the STX1A gene and is a member of the SNARE protein family critical for membrane fusion.
• Plays an essential role in hormone and neurotransmitter calcium-dependent exocytosis and endocytosis as part of the SNARE complex composed of SNAP25, STX1A, and VAMP2, which mediates synaptic vesicle fusion with the presynaptic plasma membrane. Related references: PMID:26635000
• Participates in the calcium-dependent regulation of acrosomal exocytosis in sperm, highlighting a specialized role in reproductive biology. Related references: PMID:23091057
• Involved in the exocytosis of hormones such as insulin or glucagon-like peptide 1 (GLP-1) in extra-neuronal tissues (By similarity).
• Subcellularly localized to secretory vesicle and synaptic vesicle membranes, the synapse, synaptosome, and cell membrane; also detected in secreted forms.
• Tissue expression is highest in embryonic spinal cord and ganglia, as well as adult cerebellum and cerebral cortex; also found in heart, liver, fat, skeletal muscle, kidney, and brain.
• The protein undergoes phosphorylation, ubiquitin-like conjugation, and alternative splicing; it contains a coiled-coil domain and a transmembrane helix, and is associated with Williams-Beuren syndrome.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (aa 1-100); ensure epitope accessibility by optimizing fixation/permeabilization for IF-P and IHC-P.
• In WB, expect a principal band at ~33 kDa; additional bands may reflect isoforms, post‑translational modifications, or degradation products.
• Validate the antibody in relevant human, mouse, or rat samples (e.g., neuronal tissues or cell lines) and titrate for optimal signal.
• For ELISA applications, consider pairing with a detection antibody targeting a distinct epitope; assay conditions should be empirically determined.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive IVD raw-material partner, offering validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare-target sourcing to support neuroscience and exocytosis research. For projects centered on syntaxin-1A (STX1A/Q16623), we provide scalable supply solutions and technical guidance, enabling seamless progression from discovery to clinical applications.