Anti-alpha 1 Spectrin Rabbit Monoclonal Antibody for WB & ELISA - P02549

Core Product Specifications and Parameters

Biological Background: alpha 1 Spectrin Function and Localization

• The protein encoded by the SPTA1 gene is spectrin alpha chain, erythrocytic 1, also known as erythroid alpha-spectrin. It is a key structural component of the erythrocyte membrane cytoskeleton.
• Spectrin is the major constituent of the cytoskeletal network underlying the erythrocyte plasma membrane, where it associates with band 4.1 and actin to form a superstructure that maintains cell shape and elasticity.
• The alpha spectrin chain contains multiple spectrin repeats, an SH3 domain, and a calcium-binding EF-hand motif, contributing to its ability to bind actin and organize the cortical cytoskeleton.
• Subcellularly, alpha 1 spectrin is localized to the cytoplasm, cytoskeleton, and cell cortex, consistent with its role in supporting the plasma membrane.
• Mutations in SPTA1 are associated with hereditary elliptocytosis and pyropoikilocytosis, highlighting its critical function in erythrocyte integrity.
• Post-translational modifications include phosphorylation, which may regulate spectrin interactions and cytoskeletal dynamics.
• Alternative splicing of SPTA1 produces multiple isoforms with tissue-specific expression patterns.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide mapping to an internal region (amino acids 2300–2400), suggesting the antibody recognizes a linear epitope. For Western blot, consider using a denaturing gel system and optimizing transfer conditions for high-molecular-weight proteins (~280 kDa).
• Given the predicted molecular weight of 280 kDa, a low-percentage polyacrylamide gel (e.g., 6–8%) and extended transfer times may improve detection. Include appropriate molecular weight markers to confirm band size.
• The antibody’s cross-reactivity with human, mouse, and rat indicates conservation of the epitope sequence. Validate the antibody’s performance in relevant sample lysates (e.g., erythrocyte or erythroleukemia cell lines) to confirm specificity.
• For ELISA applications, the peptide immunogen design supports direct or indirect assay formats. Optimize coating conditions and use recombinant or purified alpha 1 spectrin as a positive control when available.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a one-stop sourcing partner for IVD raw materials, connecting diagnostic developers with validated antibodies and ancillary reagents for reliable assay development. This alpha 1 Spectrin monoclonal antibody supports research into erythrocyte membrane integrity and related disorders, and CamelBio’s portfolio also includes bulk monoclonal/polyclonal antibodies, optimized antibody pairs, and customized sourcing for rare targets. Streamline your supply chain with CamelBio’s comprehensive technical and procurement support from concept to clinic.