Anti-SESN2 Rabbit Polyclonal Antibody for WB, ELISA - P58004

Core Product Specifications and Parameters

Biological Background: SESN2 Function and Localization

• SESN2 (Sestrin-2), also known as HI95, SES2, or SEST2, is a stress-inducible metabolic regulator belonging to the sestrin family.
• Functions as an intracellular leucine sensor that negatively regulates mTORC1 signaling via the GATOR complex. In leucine absence, SESN2 binds GATOR2 to inhibit mTORC1; leucine binding disrupts this interaction, activating TORC1. Related references: PMID:18692468, PMID:25263562, PMID:25457612, PMID:26449471, PMID:26586190, PMID:35114100
• Mediates TP53-dependent inhibition of TORC1 signaling in response to genotoxic stress. Related references: PMID:18692468
• Protects against oxidative stress by positively regulating NFE2L2-driven antioxidant gene expression through SQSTM1-dependent autophagic degradation of KEAP1. Related references: PMID:23274085
• May reduce reactive oxygen species (ROS) levels via intrinsic alkylhydroperoxide reductase activity of its N-terminal domain. Related references: PMID:26612684
• May suppress protein translation during endoplasmic reticulum stress by inhibiting mTORC1. Related references: PMID:24947615
• Subcellular localization is primarily cytoplasmic, with wide tissue expression. Post-translational modifications include acetylation, phosphorylation, and ubiquitination.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 211-480 of human SESN2, covering the C-terminal region. This design may aid in detecting full-length endogenous SESN2 in Western blot and ELISA applications.
• Reported cross-reactivity with mouse suggests potential utility in rodent models; validate reactivity in your specific sample system.
• For Western blot, consider using a suitable protein extraction method and loading control, as SESN2 may be induced under stress conditions.
• Optimize antibody dilution and incubation conditions for your particular assay format and sample type.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines IVD raw material procurement, providing validated antibody pairs, optimized polyclonal antibodies, and bulk ancillary reagents to diagnostic manufacturers and research labs. Our SESN2 antibody supports studies in mTOR signaling, oxidative stress, and metabolic regulation. For rare targets or custom sourcing needs, CamelBio offers a one-stop bridge from concept to clinic.