Anti-PARP1 Rabbit Monoclonal Antibody for IF/ICC, IF-P, IHC-P, ELISA - P09874

Core Product Specifications and Parameters

Biological Background: PARP1 Function and Localization

• PARP1 is a poly-ADP-ribosyltransferase that mediates PARylation of proteins, primarily on serine residues, and plays a key role in DNA repair. Related references: PMID:17177976, PMID:18055453, PMID:18172500
• It initiates repair of DNA breaks by recognizing and binding DNA breaks within chromatin, recruiting HPF1, and catalyzing histone PARylation (e.g., H2BS6ADPr, H3S10ADPr) to decompact chromatin and facilitate repair factor access. Related references: PMID:17177976, PMID:18172500, PMID:19344625
• PARP1 accumulates at DNA damage sites with TIMELESS and promotes homologous recombination repair of double-strand breaks by mediating poly-ADP-ribosylation. Related references: PMID:26344098, PMID:30356214
• Predominantly nuclear (chromatin, nucleolus), PARP1 dynamically translocates to the cytoplasm upon DNA damage or apoptosis, where it regulates processes such as cGAS-STING pathway inhibition and AIFM1-mediated apoptosis. Related references: PMID:35460603, PMID:33168626
• PARP1 modulates transcription by binding nucleosomes and altering chromatin structure like histone H1, acting as both a repressor and a context-dependent regulator of RNA polymerase II elongation. Related references: PMID:15607977, PMID:22464733, PMID:27256882, PMID:35393539
• During apoptosis, PARP1 is cleaved by caspase-3/7, generating fragments that translocate to the cytoplasm, undergo auto-poly-ADP-ribosylation, and serve as a pro-apoptotic poly-ADP-ribose carrier to induce AIFM1-mediated cell death. Related references: PMID:33168626

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide corresponding to amino acids 700–800 of human PARP1 (P09874). This region lies within the catalytic domain, which may contribute to the antibody's specificity for full-length PARP1.
• For immunofluorescence (IF/ICC and IF-P), consider using appropriate fixation and permeabilization protocols, and include known positive and negative control cell lines or tissues.
• For immunohistochemistry (IHC-P), antigen retrieval conditions and antibody dilution should be optimized empirically; use human, mouse, or rat tissues with confirmed PARP1 expression as positive controls.
• In ELISA, pairing with an appropriate detection antibody may enhance sensitivity; validate the assay with recombinant PARP1 protein or cell lysates.
• Due to the large molecular weight (113kDa), ensure optimal protein transfer conditions if using western blotting as an orthogonal method.

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CamelBio serves as your strategic one-stop partner for IVD raw-material procurement, offering a comprehensive catalog of validated antibodies, including this anti-PARP1 antibody for DNA damage and apoptosis research. We provide tailored sourcing solutions for rare-target reagents, optimized monoclonal/polyclonal antibodies, and bulk ancillary materials to streamline your diagnostic development pipeline. Connect with our team to explore custom antibody development and supply services that accelerate your path from concept to clinic.