Anti-BRMS1 Rabbit Monoclonal Antibody for WB, IHC-P, ELISA - Q9HCU9

Core Product Specifications and Parameters

Biological Background: BRMS1 Function and Localization

• BRMS1 (breast cancer metastasis-suppressor 1) is a transcriptional repressor involved in tumor suppression and metastasis inhibition.
• It down-regulates NF-kappa-B transcriptional activation by promoting deacetylation of RELA at Lys-310, which facilitates HDAC1 recruitment to target promoters.
• BRMS1 promotes apoptosis in cells with inadequate substrate adherence (anoikis), a process that prevents metastatic spread.
• The protein localizes to both the nucleus and the cytoplasm, enabling its regulatory functions in transcription and signaling.
• Expression levels are higher in term placentas than in early placentas, with generally low expression in normal and molar pregnancies.
• Structurally, BRMS1 contains a coiled-coil domain and is subject to post-translational modifications including isopeptide bond formation and ubiquitin-like conjugation.
• As a tumor suppressor, BRMS1 is identified as a key player in breast carcinoma metastasis suppression and is linked to apoptosis and transcription regulation pathways.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide corresponding to the N-terminal region (amino acids 1–100); the antibody may detect full-length BRMS1 and potentially processed forms.
• For WB, a band around 28 kDa is expected; validate specificity using positive controls such as human breast cancer cell lines (e.g., MCF-7) and relevant tissue lysates.
• For IHC-P, optimize antigen retrieval conditions and antibody dilution; consider testing on formalin-fixed paraffin-embedded tissue sections from breast carcinoma samples.
• For ELISA, the antibody can be used as a capture or detection reagent; cross-reactivity with mouse and rat orthologs allows flexible model organism studies.
• Since BRMS1 functions in NF-kB signaling and apoptosis, functional assays (e.g., luciferase reporter or anoikis assays) may complement immuno-detection.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines IVD raw-material procurement by offering validated monoclonal antibodies, bulk ancillary reagents, and rare target sourcing for diagnostic developers and researchers. For BRMS1 and related cancer biomarkers, we provide optimized antibody pairs and technical consultation to accelerate your assay development. Reach out to bridge your metastasis-suppression research with reliable, scalable raw materials.