Anti-PAK3 Rabbit Monoclonal Antibody for WB, IHC-P, ELISA - O75914

Core Product Specifications and Parameters

Biological Background: PAK3 Function and Localization

• PAK3 is a serine/threonine-protein kinase belonging to the p21-activated kinase (PAK) family.
• It acts as a downstream effector of the small GTPases CDC42 and RAC1, regulating cytoskeleton dynamics, cell migration, and cell cycle progression.
• In neurons, PAK3 plays a critical role in dendrite spine morphogenesis, synapse formation, and synaptic plasticity, partly through phosphorylation of myosin II regulatory light chain (MLC) to control actomyosin contractility.
• PAK3 is highly expressed in the nervous system, specifically in postmitotic neurons of the developing and postnatal cerebral cortex and hippocampus.
• The kinase is activated by binding to active CDC42/RAC1, leading to conformational change and autophosphorylation on multiple serine/threonine residues.
• PAK3 phosphorylates downstream targets such as MAPK4, MAPK6, and TNNI3/troponin I, linking it to MAPKAPK5-mediated F-actin polymerization and cardiac muscle contractility.
• Subcellularly, PAK3 localizes predominantly to the cytoplasm.
• Alternative splicing generates multiple isoforms; mutations in PAK3 are associated with X-linked intellectual disability.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide corresponding to the N-terminal region (aa 1-100) of human PAK3, suggesting the antibody likely recognizes an epitope within this domain, which may be conserved across mouse and rat.
• For Western blot (WB), the predicted band is ~62 kDa; consider using neuronal tissue or cell lysates (e.g., brain, hippocampus) as positive controls.
• For immunohistochemistry (IHC-P), paraffin-embedded brain sections are recommended; appropriate antigen retrieval methods should be optimized.
• ELISA can be performed with the immunogen peptide or recombinant PAK3 protein; cross-reactivity with related PAK family members should be assessed.
• Since PAK3 is expressed in both developing and adult nervous system, developmental stage-specific validation may be necessary.
• Always include appropriate negative controls (e.g., knockout cell lines or tissues) to confirm specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio empowers diagnostic manufacturers and researchers with streamlined access to high-quality IVD raw materials. For neuroscience and kinase signaling studies, we offer a curated portfolio of PAK3 antibodies, validated antibody pairs, and ancillary reagents to accelerate your assay development. Our team also specializes in sourcing rare-target raw materials, ensuring a reliable supply chain from concept to clinic.