Anti-PAR2 Rabbit Monoclonal Antibody for WB, IHC-P, ELISA - P55085

Core Product Specifications and Parameters

Biological Background: PAR2 Function and Localization

• Proteinase-activated receptor 2 (PAR2), also known as coagulation factor II receptor-like 1 (F2RL1), G protein-coupled receptor 11 (GPR11), and thrombin receptor-like 1, is a member of the G protein-coupled receptor superfamily.
• Acts as a receptor for trypsin and trypsin-like enzymes, coupling to G proteins to activate phospholipase C, intracellular calcium, MAPK, and NF-kappaB pathways.
  Related references:
  PMID:28445455
• Regulates endothelial barrier integrity during neutrophil extravasation and mediates barrier protective signaling triggered by coagulation factor Xa.
  Related references:
  PMID:23202369 PMID:22409427
• Proposed to have a bronchoprotective role in airway epithelium, but also shown to compromise the airway epithelial barrier by interrupting E-cadherin adhesion.
  Related references:
  PMID:10086357
• Promotes inflammation by signaling synergistically with TLR4 and TLR2, modulating innate and adaptive immunity, and promoting dendritic cell maturation and T-cell activation.
• Involved in cellular migration, cytoskeletal rearrangement, and chemotaxis through beta-arrestin-promoted scaffolds, promoting cofilin dephosphorylation and actin filament severing.
• Widely expressed with high levels in pancreas, liver, kidney, small intestine, and colon; expressed in endothelial cells.
  Related references:
  PMID:7556175 PMID:8615752
• Located at the cell membrane as a multi-pass transmembrane receptor, featuring disulfide bonds, palmitate modification, and N-linked glycosylation.

Experimental Guidance and Technical Tips

• The immunogen covers amino acids 298–397 in the intracellular C‑terminal tail of human PAR2, making the antibody suitable for detecting total PAR2 in denaturing (WB) and immunohistochemical (IHC-P) applications.
• For WB, run appropriate molecular weight markers and expect a band near 44 kDa; consider using a blocking peptide or knockout/knockdown lysate as a negative control.
• In IHC-P, optimize antigen retrieval for your samples and validate staining patterns in tissues with known PAR2 expression (e.g., pancreas, colon).
• For ELISA, pairing with a detection antibody that recognizes a non-overlapping epitope may improve signal specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers and laboratories a streamlined sourcing channel for IVD raw materials, including validated antibody pairs, optimized monoclonal antibodies, and bulk ancillary reagents. Our PAR2 Rabbit mAb supports inflammation, immunology, and vascular biology research, with reliable supply to accelerate your assay development. Contact us to explore custom sourcing for rare targets and comprehensive technical support.