SFRP1 Rabbit Monoclonal Antibody for WB, ELISA - Q8N474

Core Product Specifications and Parameters

Biological Background: Secreted frizzled-related protein 1 Function and Localization

• Secreted frizzled-related protein 1 (SFRP1), also known as SARP-2, is a member of the SFRP family that functions as a soluble modulator of the Wnt signaling pathway.
• SFRP1 directly interacts with Wnt ligands, antagonizing both canonical and non-canonical Wnt pathways; it decreases intracellular β-catenin levels and inhibits WNT1/WNT4-mediated TCF-dependent transcription.
• By regulating Wnt signaling, SFRP1 plays critical roles in cell growth, differentiation, and apoptosis; it exhibits antiproliferative effects on vascular cells, delaying G1/S phase progression, and can induce angiogenic responses in vivo.
• In kidney development, SFRP1 inhibits tubule formation and bud growth in metanephroi, highlighting its importance in organogenesis.
• SFRP1 is a secreted glycoprotein containing a cysteine-rich domain and disulfide bonds, characteristic of frizzled-related proteins.
• It is expressed broadly, with highest levels in heart and fetal kidney, and also in testis, ovary, fetal brain, vascular smooth muscle cells, among others; notably absent in lung, liver, and peripheral blood leukocytes.

Experimental Guidance and Technical Tips

• The antibody was raised against an N-terminal peptide (aa 1-100) of human SFRP1, suggesting reactivity with the full-length ~35 kDa protein and potential isoforms or fragments containing this region; consider performing epitope mapping if isoform specificity is required.
• For Western blotting, validate the antibody in your specific cell or tissue lysates; SFRP1 is a secreted protein, so using conditioned medium or optimizing lysis buffers for secreted proteins may improve detection.
• Select appropriate positive controls such as heart or fetal kidney lysates, given the higher expression levels in these tissues; negative controls like lung or liver may help confirm specificity.
• In ELISA applications, pairing with a suitable capture/detection antibody may be required; test serial dilutions to determine the optimal working concentration for your assay format.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic and research communities with a comprehensive portfolio of IVD-grade antibodies, including validated monoclonal antibodies like this SFRP1 mAb, suitable for Wnt signaling and developmental biology applications. We provide ready-to-use antibody pairs, bulk reagent supply, and custom sourcing for rare or emerging targets to accelerate your assay development from concept to clinic.